Cat.No. : CSC-RO0875 Host Cell: HEK293T
| Cat. No. | CSC-RO0875 |
| Description | This cell line is engineered to stably overexpress human TREM2-TYROBP (also named as TREM2-DAP12). |
| Gene | TREM2-TYROBP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: To demonstrate the physiological relevance of the Human TREM2-TYROBP Stable Cell Line - HEK293T in neuroinflammatory processes, researchers can utilize in vitro co-culture systems with microglia and neuronal cells to observe the interactions and effects of TREM2-TYROBP signaling on neuroinflammation. Additionally, creating a brain-on-a-chip model that incorporates the cell line could provide insights into the cell signaling dynamics under conditions mimicking the brain microenvironment. These models can help elucidate the role of TREM2-TYROBP in microglial activation and its potential impact on neurodegenerative diseases.
A: The overexpression of TREM2 and TYROBP in the Human TREM2-TYROBP Stable Cell Line - HEK293T enhances the cell's capacity for amyloid-beta phagocytosis in vitro, mimicking a key function of microglia in the Alzheimer's disease (AD) brain. This effect could facilitate the identification of potential therapeutic targets or compounds that modulate TREM2-TYROBP signaling, aiming to boost amyloid-beta clearance. Such findings have profound implications for Alzheimer's research, offering a pathway to developing treatments that might slow or reverse the progression of the disease by enhancing the natural defense mechanisms of the brain.
A: Yes, the Human TREM2-TYROBP Stable Cell Line - HEK293T is an excellent tool for screening molecules that modulate the TREM2-TYROBP signaling pathway. Optimal assay designs for this application include the use of reporter gene assays, where activation or inhibition of the TREM2-TYROBP pathway can be quantitatively measured via luciferase or GFP under the control of NF-κB or AP-1 promoters, which are downstream of TYROBP. Such assays can efficiently identify small molecules or antibodies that influence this signaling pathway, thereby contributing to the development of novel therapeutics for diseases associated with altered microglial function.
A: Ensuring the successful transfection and stable expression of TREM2 and TYROBP in the HEK293T cell line involves optimizing transfection conditions, including the choice of transfection reagent, DNA concentration, and cell density. Selecting a suitable antibiotic for selection and maintaining cells under selection pressure for an adequate period are crucial to achieve stable expression. Regular verification of gene expression through qPCR or Western blot is necessary to confirm the stability of the expression over time. These technical considerations are vital for maintaining the cell line's reliability and reproducibility in research, especially when studying the biological functions of TREM2 and TYROBP in neurodegeneration and immune response.
A: The Human TREM2-TYROBP Stable Cell Line - HEK293T advances our understanding of molecular mechanisms underlying microglial activation by providing a consistent and reproducible model to study the TREM2-TYROBP signaling axis. By manipulating the expression of TREM2 and TYROBP in this cell line and observing the resultant changes in signaling pathways, researchers can dissect the roles these molecules play in microglial activation, phagocytosis, and secretion of inflammatory mediators. This insight is crucial for neurodegenerative disease research, where aberrant microglial activation is often implicated in disease progression. Through such studies, the cell line helps identify potential therapeutic targets to modulate microglial function and ameliorate neurodegenerative disease symptoms.
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The Human TREM2-TYROBP Stable Cell Line - HEK293T facilitates enhanced analysis of the TREM2-TYROBP signaling pathway, crucial in immune response and neuroinflammatory processes. This cell line allows us to study the complex interactions and signaling mechanisms between TREM2 and TYROBP, providing insights into their roles in diseases like Alzheimer's.
This cell line provides a robust platform for studying the functional interactions between TREM2 and TYROBP. The Human TREM2-TYROBP Stable Cell Line - HEK293T allows for detailed examination of how these proteins cooperate to modulate immune functions in the brain, which is critical for developing therapeutic strategies.
By providing a consistent expression system for both TREM2 and TYROBP, this cell line is an excellent resource for validating potential therapeutic targets. We can use the Human TREM2-TYROBP Stable Cell Line - HEK293T to test the efficacy of compounds that influence TREM2 and TYROBP activity, aiding in drug development.
The robust nature of the HEK293T cell line, combined with the stable expression of TREM2 and TYROBP, makes it highly adaptable for a variety of biochemical and cellular assays. This adaptability ensures that the Human TREM2-TYROBP Stable Cell Line - HEK293T can be used effectively across a range of experimental conditions.
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