Cat.No. : CSC-RO0902 Host Cell: CHO-K1
| Cat. No. | CSC-RO0902 |
| Description | This cell line is engineered to stably overexpress human TREM2-TYROBP (also named as TREM2-DAP12). |
| Gene | TREM2-TYROBP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human TREM2-TYROBP Stable Cell Line - CHO-K1 is a cell line developed through the stable expression of human Triggering Receptor Expressed on Myeloid cells 2 (TREM2) and TYRO protein tyrosine kinase binding protein (TYROBP). TREM2 and TYROBP play significant roles in the pathogenesis of Alzheimer's Disease (AD), particularly in processes related to inflammatory responses and neuronal degeneration. Therefore, this cell line can be used to study how TREM2 and TYROBP influence the progression of Alzheimer's Disease, particularly in terms of inflammation signaling, amyloid-beta deposition, and neuroprotection.
A: When using this cell line for drug screening, it is crucial to assess the effects of drugs on the expression of TREM2 and TYROBP and their downstream signaling pathways. This can be evaluated by measuring the release of inflammatory factors, production and processing of amyloid-beta, and the expression of neuroprotective factors. Additionally, molecular biology and cell imaging techniques, such as flow cytometry and confocal microscopy, can be used to observe the drug's impact on these pathological processes more precisely.
A: To simulate the Alzheimer's Disease microenvironment, various stimuli such as amyloid-beta and inflammatory cytokines can be added to Human TREM2-TYROBP Stable Cell Line - CHO-K1. This allows for observation of the response and regulatory roles of TREM2 and TYROBP under such conditions. By evaluating cell viability, proliferative capacity, production of inflammatory factors, and processing of amyloid-beta, a better understanding of the cell line's performance in an Alzheimer's Disease-like microenvironment can be gained.
A: To study the role of TREM2 and TYROBP in neuroinflammation using Human TREM2-TYROBP Stable Cell Line - CHO-K1, inflammatory stimuli like lipopolysaccharides can be added to induce an inflammatory response, followed by assessing the impact of TREM2 and TYROBP on inflammatory signaling pathways. The release of inflammatory cytokines such as TNF-α and IL-6, and indicators of cellular stress responses, like the production of Reactive Oxygen Species (ROS) and the expression of antioxidant enzymes, can be measured.
A: Human TREM2-TYROBP Stable Cell Line - CHO-K1 can be used to study the impact of TREM2 gene mutations on the pathogenesis of Alzheimer's Disease (AD). By introducing specific TREM2 mutations using gene editing technologies like CRISPR/Cas9, genetic variations associated with AD can be simulated. Further, it allows for the investigation of how these mutations affect the functions of TREM2 and TYROBP, including their impact on amyloid-beta processing, regulation of inflammatory responses, and interactions with other cell types.
A: Although primarily used for studying the pathological mechanisms related to Alzheimer's Disease, Human TREM2-TYROBP Stable Cell Line - CHO-K1 can also be employed to explore the roles of TREM2 and TYROBP in other diseases. For instance, due to the involvement of these proteins in immune regulation and inflammatory responses, this cell line can be used in research on autoimmune diseases, chronic inflammatory disorders, and certain types of cancers.
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