Human WRN Knockout Cell Line-HEK293T

Name: Human WRN Knockout Cell Line-HEK293T
SKU: CSC-RT2775
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2775

Target Gene : WRN Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2775
Description	This cell is a stable cell line with a homozygous knockout of human WRN using CRISPR/Cas9.
Target Gene	WRN
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	WRN Werner syndrome, RecQ helicase-like [ Homo sapiens ]
Gene Symbol	WRN
Synonyms	RECQ3; RECQL2; RECQL3
GeneID	7486
Uni ProtID	Q14191
mRNA Refseq	NM_000553.4
Protein Refseq	NP_000544.2
Chromosome Location	8p12
Function	3-5 DNA helicase activity; 3-5 exonuclease activity; ATP binding; ATP-dependent 3-5 DNA helicase activity; ATP-dependent DNA helicase activity; ATPase activity; DNA binding; DNA helicase activity; DNA helicase activity; G-quadruplex DNA binding; Y-form DNA binding; bubble DNA binding; exonuclease activity; four-way junction helicase activity; helicase activity; magnesium ion binding; manganese ion binding; protein binding; protein complex binding; protein homodimerization activity;
Pathway	Integrated Pancreatic Cancer Pathway, organism-specific biosystem; Regulation of Telomerase, organism-specific biosystem;
MIM	604611

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Background

Applications

Q & A

Customer Reviews

The WRN gene, also known as Werner syndrome RecQ-like helicase (RECQL2, RECQL3, RECQ3), is a key human gene involved in maintaining genome integrity. It is located on chromosome 8 and encodes Werner protein, which plays an important role in DNA repair and replication. Werner protein primarily functions as a helicase, an enzyme that unwinds and separates double-stranded DNA. In addition, it acts as an exonuclease, trimming damaged DNA 3' ends. These dual functions enable Werner protein to resolve complex DNA structures and ensure accurate DNA replication and repair.

Mutations in the WRN gene cause Werner syndrome, a disease characterized by premature aging and increased risk of cancer. More than 60 different mutations are known to cause Werner syndrome, which usually result in a truncated and non-functional Werner protein. This defective protein is unable to localize to the cell nucleus and perform its critical role, resulting in disruptions in the DNA repair, replication, and transcription processes. As a result, cells accumulate DNA damage over time, accelerating the aging process and predisposing individuals to a variety of age-related diseases.

Cancer Research: Werner syndrome is linked to premature aging and increased cancer susceptibility. By using WRN knockout HEK293T cells, researchers can study the mechanisms by which WRN deficiency contributes to genomic instability and cancer development.

Aging Studies: WRN is involved in DNA repair and maintaining genomic stability. The knockout cell line is invaluable for understanding the molecular pathways of aging and identifying new strategies to combat age-related diseases.

DNA Repair Mechanisms: Since WRN plays a substantial role in DNA repair, the WRN knockout HEK293T cells are used to investigate the specific pathways and mechanisms impacted by WRN deficiency. This can aid in developing therapies for diseases caused by defective DNA repair mechanisms.

Drug Testing and Development: The WRN knockout cell line can be used to screen for drugs that might enhance the repair of DNA or protect against DNA damage. This can be particularly useful for developing treatments for cancer and age-related diseases.

Model for Genetic Disorders: HEK293T cells with WRN knockout can serve as a model for Werner syndrome, a genetic disorder characterized by premature aging. This enables better understanding of the disease and helps in developing potential therapies.

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Great!

Great! By using WRN knockout cells, we have made significant progress in understanding the underlying mechanisms of Werner syndrome and potentially identifying new treatment strategies.

United States

2022-07-19

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Human WRN Knockout Cell Line-HEK293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Great!

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