Human TLR2 Stable Cell Line - BaF3

Name: Human TLR2 Stable Cell Line - BaF3
SKU: CSC-RO1015

Cat.No. : CSC-RO1015 Host Cell: BaF3

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO1015
Description	This cell line is engineered to stably overexpress human toll like receptor 2toll like receptor 2 (TLR2) in BaF3.
Gene	TLR2
Gene Species	Homo sapiens (Human)
Host Cell	BaF3
Host Cell Species	Mus musculus (Mouse)
Stability	Validated for at least 10 passages
Application	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

How to ensure the specificity of TLR2 activation signals in Human TLR2 Stable Cell Line - BaF3 activation experiments?

A: Use specific TLR2 agonists or ligands, and set appropriate negative controls such as wild-type BaF3 cells, to ensure the observed activation signals are specific.

How to address the slow cell growth in cell proliferation experiments with Human TLR2 Stable Cell Line - BaF3?

A: Optimize culture conditions, such as adjusting the composition and temperature of the culture medium, ensure appropriate concentrations of serum and growth factors, and avoid over-passaging.

How to enhance the detection signal of cell surface markers in flow cytometry analysis of Human TLR2 Stable Cell Line - BaF3?

A: Use high-affinity and specificity antibodies, optimize the antibody dilution ratio and incubation time, and ensure cell viability during processing and analysis.

How to improve CRISPR/Cas9 gene editing efficiency in Human TLR2 Stable Cell Line - BaF3?

A: Optimize the design of sgRNA, improve the expression efficiency of Cas9, ensure the accessibility of the target sequence, and use efficient transfection methods.

How to improve the detection efficiency of specific protein interactions in co-immunoprecipitation experiments with Human TLR2 Stable Cell Line - BaF3?

A: Optimize cell lysis conditions, use appropriate immunoprecipitation reagents and antibodies for TLR2 protein, ensure sufficient incubation time, and proper washing steps.

How to enhance the sensitivity of Human TLR2 Stable Cell Line - BaF3 cells to drugs in drug screening experiments?

A: Adjust drug treatment concentration and duration to match the cell state, and optimize cell density and culture conditions if necessary.

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