Cat.No. : CSC-RR0518 Host Cell: THP-1
| Cat. No. | CSC-RR0518 |
| Description | This cell line is engineered to stably overexpress GFP and luciferase reporter genes. THP-1 is a human leukemia monocytic cell line, which has been widely used in laboratory research. This cell line is a useful tool for fluorescent tracking of THP-1 cells. |
| Gene | GFP/Luciferase |
| Host Cell | THP-1 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: GFP/Luc Reporter THP-1 Cell Line is a modified version of the THP-1 human monocytic cell line that has been engineered to express both green fluorescent protein (GFP) and luciferase. This cell line is useful for studying gene expression and protein interactions, as the GFP allows for visual tracking of protein localization, while luciferase provides a quantifiable readout of gene expression levels.
A: The GFP/Luc Reporter THP-1 Cell Line is invaluable in molecular biology research as it enables the visualization and quantification of specific protein expression and interactions in real-time. This cell line can be used to study various cellular processes, such as signal transduction, protein trafficking, and gene regulation, providing a powerful tool for deciphering the complexities of cellular events.
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