GFP/Luc Reporter Cell Line - THP-1

Name: GFP/Luc Reporter Cell Line - THP-1
SKU: CSC-RR0518
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0518

Host Cell : THP1 Size : >1x10⁶ frozen cells/vial

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Cat. No.	CSC-RR0518
Description	This cell line is engineered to stably overexpress GFP and luciferase reporter genes. THP-1 is a human leukemia monocytic cell line, which has been widely used in laboratory research. This cell line is a useful tool for fluorescent tracking of THP-1 cells.
Target Gene	GFP/Luciferase
Host Cell	THP1
Host Cell Species	Homo sapiens (Human)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RR0518-GFP/Luc Reporter Cell Line - THP-1-Datasheet

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Background

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The GFP/Luc Reporter gene is a fusion gene that combines the green fluorescent protein (GFP) gene with the Luc gene, which encodes the luciferase enzyme. This reporter gene is commonly used in molecular biology to study gene expression and regulation. It allows researchers to visualize and quantify the expression of a specific gene of interest by monitoring the fluorescence or bioluminescence signal emitted by the fusion protein.

The THP-1 Cell Line is a human monocytic cell line that has been widely used in biomedical research. It was derived from the monocytes of a patient with monocytic leukemia. THP-1 cells are immortalized and can be cultured in vitro under standard laboratory conditions. They have been employed in various studies, including the investigation of inflammation, infection, and cell signaling pathways.

GFP/Luc Reporter THP-1 Cell Line is a dual reporter cell line that combines the advantages of the GFP and luciferase reporter genes. The cell line is derived from the human monocyte cell line THP-1 and has been modified to stably express both GFP and luciferase genes under the control of the relevant promoters.One of the main applications of the GFP/Luc Reporter THP-1 Cell Line is the study of gene regulation and signalling pathways. By using this cell line, researchers can simultaneously monitor gene expression and promoter activity to gain a comprehensive understanding of the molecular mechanisms underlying these processes. In addition, this cell line can be used to study the effects of various stimuli such as hormones, growth factors and drugs on gene expression and signalling pathways. In addition, the GFP/Luc reporter THP-1 cell line , can be used in high-throughput screening assays to identify novel compounds that modulate gene expression or signalling pathways. In summary, the GFP/Luc Reporter THP-1 Cell Line is a powerful tool for a wide range of applications in molecular and cellular biology, including the study of gene regulation, signalling pathways and high-throughput screening.

Customer Q&As

What is GFP/Luc Reporter THP-1 Cell Line?

A: GFP/Luc Reporter THP-1 Cell Line is a modified version of the THP-1 human monocytic cell line that has been engineered to express both green fluorescent protein (GFP) and luciferase. This cell line is useful for studying gene expression and protein interactions, as the GFP allows for visual tracking of protein localization, while luciferase provides a quantifiable readout of gene expression levels.

How is GFP/Luc Reporter THP-1 Cell Line useful in molecular biology research?

A: The GFP/Luc Reporter THP-1 Cell Line is invaluable in molecular biology research as it enables the visualization and quantification of specific protein expression and interactions in real-time. This cell line can be used to study various cellular processes, such as signal transduction, protein trafficking, and gene regulation, providing a powerful tool for deciphering the complexities of cellular events.

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