Vaccinia Capping Enzyme

RSNK-022

Vaccinia Capping Enzyme is a recombinant protein from Vaccinia virus. Vaccinia Capping Enzyme can add 7-methylguanylate cap structure (Cap 0) to the 5' end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, translation of mRNAs. Vaccinia Capping enzyme is composed of two subunits (D1R, D12L), and has three enzymatic activities (RNA triphosphatase, guanylyltransferase by the D1R subunit and guanine methyltransferase by the D12L subunit). All necessary for addition of a complete Cap 0 structure, m7Gppp5'N). In vitro, transcripts can be capped in the presence of reaction buffer, GTP, and the methyl donor (SAM).

• Labeling 5´ end of mRNA.
• Capping mRNA prior to translation assays/in vitro translation.

10X VCE Capping Buffer

25U/μL

• Convenient solution that includes all components required for Enzymatic capping System based on the Vaccinia virus Capping Enzyme (VCE).
• Isolated form a recombinant source.
• Extensively quality controlled: Endo/Exonucleases/RNAses free.

>95%

Quality Test includs: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

500U, 1000U, 2000U, 2500U

Recombinant E.coli

-20°C