Faustovirus Capping Enzyme

Name: Faustovirus Capping Enzyme
SKU: RSNK-021
Rating: 4.0 (1 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

RSNK-021

Description

The 7-methylguanosine cap structure (m7Gppp, Cap0) is a specific feature in eukaryotic mRNA, which is required for mRNA stability, splicing, nuclear export, effective translation, and mRNA decay. Installation of Cap-0 is a key step in eukaryotic mRNA maturation along with 2′-O-methylation at position 1 (Cap-1) and polyadenosine (poly(A)) tailing. The Cap-0 structure also promotes RNA stability and prevents inadvertent activation of innate immune responses triggered by triphosphorylated RNA. Faustovirus Capping Enzyme is a single-subunit enzyme that combines the three activities necessary to produce the Cap-0 structure- triphosphatase, guanylyltransferase, and (guanine-N7)-methyltransferase.

Applications

• Labeling 5´ end of mRNA.
• Capping mRNA prior to translation assays/in vitro translation.

Buffer

10X FCE Capping Buffer

Concentration

25U/μL

Features

• Convenient solution that includes all components required for Enzymatic capping System based on the Faustovirus Capping Enzyme (FCE).
• Isolated form a recombinant source.
• Extensively quality controlled: Endo/Exonucleases/RNAses free.
• Enable one-pot Cap-1 synthesis, as FCE is compatible with mRNA Cap 2′-O-Methyltransferase

Purity

>95%

Quality

Quality Test includs: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

Size/Form

500U, 1000U, 2000U, 2500U

Source

Recombinant E.coli

Storage

-20°C

Publications

Q & A

Customer Reviews

Customer Q&As

What is the mechanism of action of T7 polymerase?

A: The T7 polymerase is an RNA polymerase that catalyzes the synthesis of RNA from a DNA template. It has a high specificity for promoters containing a T7 phage consensus sequence.

Where is T7 RNA polymerase found?

A: T7 RNA polymerase is a type of RNA polymerase that is isolated from the T7 bacteriophage, a virus that infects Escherichia coli (E. coli) bacteria.

What are the applications of T7 DNA polymerase?

A: The high processivity of T7 RNA Polymerase holds tremendous potential in applications related to DNA sequencing and synthesis. In DNA sequencing, it plays an essential role in the preparation of RNA libraries, where its ability to produce homogeneous transcription intermediates ensures high fidelity of sequencing data. For DNA synthesis, the processive synthesis of complimentary RNA can be programmed to generate a virtually unlimited number of sequence permutations.

What is the stability of T7 RNA polymerase?

A: T7 RNA polymerase is relatively stable and can withstand a range of temperatures and pH conditions. It exhibits high stability at temperatures up to 40-42°C, allowing it to function optimally under these conditions.

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Customer Reviews

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The T7 RNA Polymerase is widely used for the synthesis of specific transcripts from DNA in the 5→ 3 direction, as well as being a suitable model for studying the mechanisms of transcription.

United States

02/23/2023

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Faustovirus Capping Enzyme

Publications

Q & A

Customer Reviews

What is the mechanism of action of T7 polymerase?

Where is T7 RNA polymerase found?

What are the applications of T7 DNA polymerase?

What is the stability of T7 RNA polymerase?

Invaluable tool

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