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T4 DNA Ligase

Cat.No.
EMQZ1404
Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Source Description
Purified from a recombinant E. coli strain.
Applications
• Cloning of restriction fragments • Joining linkers and adapters to blunt-ended DNA
Unit Definition
One unit is the amount of enzyme required to yield 50% ligation of HindIII fragments of lambda DNA in 30 min at 16 °C in a 20 μl assay mixture containing a DNA terminus concentration of 0.12 μM.
Reaction Conditions
1×T4 DNA Ligase Reaction Buffer [50 mM Tris-HCl (pH 7.5 at 25°C), 10 mM MgCl2, 10 mM DTT, 1 mM ATP; incubate at 16°C.
Concentration
400,000 U/ml
Size
20,000 U; 100,000 U
Storage Conditions
Store at -20°C

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