T4 RNA Ligase

EMQZ1417

T4 RNA Ligase catalyzes the formation of a phosphodiester bond between a 5´-phosphoryl-terminated nucleic acid donor and a 3´-hydroxyl-terminated nucleic acid acceptor in a template-independent manner. The enzyme is ATP-dependent, and is active on a broad range of substrates including RNA, DNA, oligoribonucleotides, oligodeoxyribonucleotides, as well as numerous nucleotide derivatives.

• RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate• Joining RNA to RNA• Synthesis of oligoribonucleotides and oligodeoxyribonucleotides• Specific modifications of tRNAs• Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends)• Site-specific generation of composite primers for PCR

10,000 U/ml

500 U; 5,000 U

E. coli strain

Store at -20°C