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T4 RNA Ligase

Cat.No.
EMQZ1417
Description
T4 RNA Ligase catalyzes the formation of a phosphodiester bond between a 5´-phosphoryl-terminated nucleic acid donor and a 3´-hydroxyl-terminated nucleic acid acceptor in a template-independent manner. The enzyme is ATP-dependent, and is active on a broad range of substrates including RNA, DNA, oligoribonucleotides, oligodeoxyribonucleotides, as well as numerous nucleotide derivatives.
Source Description
E. coli strain
Applications
• RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate • Joining RNA to RNA • Synthesis of oligoribonucleotides and oligodeoxyribonucleotides • Specific modifications of tRNAs • Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends) • Site-specific generation of composite primers for PCR
Concentration
10,000 U/ml
Size
500 U; 5,000 U
Storage Conditions
Store at -20°C

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