T4 RNA Ligase

Name: T4 RNA Ligase
SKU: EMQZ1417
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1417

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Product Information

Cat. No.	EMQZ1417
Description	T4 RNA Ligase catalyzes the formation of a phosphodiester bond between a 5´-phosphoryl-terminated nucleic acid donor and a 3´-hydroxyl-terminated nucleic acid acceptor in a template-independent manner. The enzyme is ATP-dependent, and is active on a broad range of substrates including RNA, DNA, oligoribonucleotides, oligodeoxyribonucleotides, as well as numerous nucleotide derivatives.
Source	E. coli strain
Concentration	10,000 U/ml
Applications	• " • RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate • Joining RNA to RNA • Synthesis of oligoribonucleotides and oligodeoxyribonucleotides • Specific modifications of tRNAs • Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends) • Site-specific generation of composite primers for PCR"
Size	500 U; 5,000 U
Storage	Store at -20°C

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Q & A

Customer Reviews

Customer Q&As

Is this ligase ATP-dependent?

A: Yes, the enzyme is ATP-dependent.

Can T4 RNA Ligase only be used to connect RNA to RNA?

A: T4 RNA Ligase can be used to connect RNA to RNA, RNA to DNA, and DNA to DNA. However, the efficiency of the latter two connections is low.

How long does the reaction usually take when using this enzyme to connect RNA to RNA?

A: Generally, the reaction is performed at 16°C for 2 hours or overnight.

Where does the enzyme come from?

A: The expression gene of the enzyme comes from T4 phage, and the enzyme is expressed and purified from E. coli.

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Customer Reviews

High activity

The ligase has activity on a wide range of substrates, including RNA, DNA, oligonucleotides, deoxyoligonucleotides, and many nucleotide derivatives.

United States

2022-01-31

Fast delivery

Generally, the reagents can be received within a week.

United States

2022-08-05

RNA modification

T4 RNA Ligase can be used to generate site-specifically modified RNAs, especially oligonucleotide modifications and anticodon tRNA modifications.

United States

2023-03-15

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T4 RNA Ligase

Product Information

Q & A

Customer Reviews

Is this ligase ATP-dependent?

Can T4 RNA Ligase only be used to connect RNA to RNA?

How long does the reaction usually take when using this enzyme to connect RNA to RNA?

Where does the enzyme come from?

High activity

Fast delivery

RNA modification

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