T4 RNA Ligase catalyzes the formation of a phosphodiester bond between a 5´-phosphoryl-terminated nucleic acid donor and a 3´-hydroxyl-terminated nucleic acid acceptor in a template-independent manner. The enzyme is ATP-dependent, and is active on a broad range of substrates including RNA, DNA, oligoribonucleotides, oligodeoxyribonucleotides, as well as numerous nucleotide derivatives.
E. coli strain
• RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate
• Joining RNA to RNA
• Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
• Specific modifications of tRNAs
• Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends)
• Site-specific generation of composite primers for PCR