Cat.No. : CSC-RT0560
Host Cell: Hela Target Gene: IGF1R
Size: >1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0560 |
| Description | This cell line is a stable cell line with a homozygous knockout of human IGF1R using CRISPR/Cas9. |
| Target Gene | IGF1R |
| Gene ID | 3480 |
| Genotype | IGF1R (-/-) |
| Host Cell | Hela |
| Host Cell Species | Homo sapiens (Human) |
| Cell Type | Epithelial |
| Size | >1x10^6 cells/vial, 1mL |
| SequencingResult | Homozygous: 100 bp deletion in exon |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: DMEM supplemented with 10% fetal bovine serum.
It is not required to add the selection antibiotics when culturing the KO cells.
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
IGF1R knockout cells offer a platform for the screening of drugs or compounds that target the IGF-1 signaling pathway or related pathways. I recommend it.
Great buy! By using the Creative BIogene's IGF1R knockout cell, we can evaluate their specificity and potency in disrupting the IGF-1 signaling pathway.
The shipping process for the Human IGF1R Knockout Cell Line-HeLa was quick and efficient. The cells arrived in excellent condition, well-packaged, and ready for use. We appreciate the effort put into ensuring the safe transportation of this valuable resource.
The Human IGF1R Knockout Cell Line-HeLa has significantly advanced our research, enabling us to study the function of IGF1R in a more detailed manner.
The cell line's viability and cell death rates have been exceptional, leading to accurate and reliable.
The Human IGF1R Knockout Cell Line-HeLa has seamlessly integrated into our existing experimental workflow, thanks to the comprehensive documentation provided.
We appreciate the effort to ensure the ethical sourcing of the Human IGF1R Knockout Cell Line-HeLa, aligning with our research ethics.
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