Human BRCA2 Knockdown Cell Line-HeLa

Name: Human BRCA2 Knockdown Cell Line-HeLa
SKU: CSC-RK0027
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RK0027

Host Cell : HeLa Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-RK0027
Background	Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.
Target Gene	BRCA2
Abbr	HeLa-HuBRCA2 Knockdown
Alias	BRCA2, FACD, GLM3, FANCB, FANCD, PNCA2
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Morphology	Epithelial
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Shipping	Dry ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BRCA2 breast cancer 2, early onset [ Homo sapiens ]
Gene Symbol	BRCA2
Synonyms	FAD; FACD; FAD1; GLM3; BRCC2; FANCB; FANCD; PNCA2; FANCD1; BROVCA2
Gene Description	breast cancer 2, early onset
GeneID	675
Uni ProtID	P51587
mRNA Refseq	NM_000059.3
Protein Refseq	NP_000050.2
Chromosome Location	13q12.3
Function	H3 histone acetyltransferase activity; H4 histone acetyltransferase activity; gamma-tubulin binding; NOT histone acetyltransferase activity; protease binding; protein binding; single-stranded DNA binding;
Pathway	DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; FOXM1 transcription factor network, organism-specific biosystem; Fanconi Anemia pathway, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Homologous DNA pairing and strand exchange, organism-specific biosystem;
MIM	600185

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