Human BRCA2 Knockout Cell Line-DLD-1

Name: Human BRCA2 Knockout Cell Line-DLD-1
SKU: CSC-RT0028
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0028

Target Gene : BRCA2 Host Cell : DLD-1

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0028
Description	DLD-1-BRCA2 (+/-) is a cell line with a heterozygous knockout of human BRCA2
Target Gene	BRCA2
Host Cell	DLD-1
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BRCA2 breast cancer 2, early onset [ Homo sapiens ]
Gene Symbol	BRCA2
Synonyms	FAD; FACD; FAD1; GLM3; BRCC2; FANCB; FANCD; PNCA2; FANCD1; BROVCA2
Gene Description	breast cancer 2, early onset
Gene ID	675
Uni Prot ID	P51587
m RNA Refseq	NM_000059.3
Protein Refseq	NP_000050.2
Chromosome Location	13q12.3
Function	H3 histone acetyltransferase activity; H4 histone acetyltransferase activity; gamma-tubulin binding; NOT histone acetyltransferase activity; protease binding; protein binding; single-stranded DNA binding;
Pathway	DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; FOXM1 transcription factor network, organism-specific biosystem; Fanconi Anemia pathway, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Homologous DNA pairing and strand exchange, organism-specific biosystem;
MIM	600185

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Prefectly knockout

Research BRCA2 gene, this product can perfectly knock out human BRCA2 gene, high efficiency and low cost

United States

2021-12-02

Easy to test

Samples can be tested in a variety of simple experiments, greatly speeding up the process.

United States

2022-02-06

Convinced

This product is easy to handle and requires essentially no fuss, but its results are very clear and very convincing.

United States

2022-09-10

Convinced

This product is easy to handle and requires essentially no fuss, but its results are very clear and very convincing.

United States

2023-02-08

Speed up the process

This transfection was more successful than the previous experimental materials, and I couldn't help but feel that this reagent was so amazing, and my experimental process was one step faster.

United States

2021-12-02

Repeat good result

In this product, gene knockout is such a simple thing, and the results of repeated experimental experiments are also very clear.

United States

2022-02-06

Will use again

In my follow-up experiments, there will also be gene knockout experiments, I will give priority to this brand of transfection reagent, there is a first surprise, I want to have the next time.

United States

2022-09-10

Efficient

I'm so proud of my experimental results, and I think the knockout transfection reagent I used is a big contribution.

United States

2023-01-18

Effect strong

This is not the first time I have used this brand's products, and the second time I have used it, the effect is still very strong.

United States

2023-02-21

Worked very well

Having used trial packs of other reagents from this company before, this is the first time I ordered a gene knockout reagent, and it worked very well.

United States

2023-03-05

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Human BRCA2 Knockout Cell Line-DLD-1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Prefectly knockout

Easy to test

Convinced

Convinced

Speed up the process

Repeat good result

Will use again

Efficient

Effect strong

Worked very well

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