Elastase I-SaCas9 AAV (Serotype 9)

Elastase I-SaCas9 AAV (serotype 9) is an advanced tool in the field of genetic engineering, especially for genome editing applications. This adeno-associated virus (AAV) particle is designed to express the CRISPR-associated protein 9 (Cas9) derived from Staphylococcus aureus, or SaCas9. It is driven by the Elastase I promoter, a key regulatory element known for its ability to ensure tissue-specific gene expression, especially in pancreatic cells. Expression systems driven by the Elastase I promoter allow researchers to target specific cell types, which is critical for studies that require precise gene editing in target tissues. AAV, especially serotype 9, is known for its efficient transduction in a variety of tissues, including muscle, liver, and the central nervous system, due to its strong ability to penetrate the blood-brain barrier. The compact nature of SaCas9 is particularly advantageous compared to the more commonly used SpCas9 (derived from Streptococcus pyogenes), as it can be more easily accommodated within the packaging constraints of AAV vectors, allowing for the inclusion of additional genetic elements such as guide RNA (gRNA) or other regulatory sequences. The smaller size not only facilitates easy packaging into AAV vectors but also enhances the co-delivery of Cas9 and gRNA, thus simplifying the gene editing process.