mInsulin2-SaCas9 AAV (Serotype 9)

The mInsulin2-SaCas9 adeno-associated virus (AAV) serotype 9 is a scientifically engineered viral vector designed for gene editing applications. This pre-made AAV particle utilizes the Staphylococcus aureus Cas9 protein (often abbreviated as SaCas9), a smaller Cas9 ortholog that has high efficiency and fidelity in gene editing tasks. SaCas9 is smaller than the traditional SpCas9 and can therefore be packaged more efficiently in AAV vectors, enabling the co-delivery of additional regulatory elements when needed. SaCas9 is regulated by the mInsulin2 promoter. The mInsulin2 promoter refers to a specific DNA sequence that regulates the expression of the mouse insulin 2 gene. Promoters are critical DNA regions located upstream of a gene; they serve as binding sites for transcription factors and other proteins that control the initiation of transcription. In this context, the mInsulin2 promoter is responsible for controlling when and how much insulin the insulin 2 (Ins2) gene produces in response to various physiological signals. AAV serotype 9 has good tropism and can efficiently transduce a variety of cell types, especially in neural tissue and muscle, making AAV9 vectors very suitable for systemic and local delivery. This special vector design is suitable for studying gene function and regulation, which is expected to bring new insights into therapeutic development, especially in the field of endocrine and metabolic research affecting diabetes and related diseases.