Panoply™ Human NTRK2 Over-expressing Stable Cell Line

Name: Panoply™ Human NTRK2 Over-expressing Stable Cell Line
SKU: CSC-SC010683
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC010683

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC010683
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	NTRK2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NTRK2 neurotrophic tyrosine kinase, receptor, type 2 [ Homo sapiens ]
Gene Symbol	NTRK2
Synonyms	TRKB; trk-B; GP145-TrkB
Gene Description	neurotrophic tyrosine kinase, receptor, type 2
Gene ID	4915
Uni Prot ID	Q16620
m RNA Refseq	NM_006180.3
Protein Refseq	NP_006171.2
Chromosome Location	9q22.1
Function	ATP binding; brain-derived neurotrophic factor binding; brain-derived neurotrophic factor-activated receptor activity; neurotrophin binding; neurotrophin binding; protein homodimerization activity;
Pathway	Activation of TRKA receptors, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; NGF signalling via TRKA from the plasma membrane, organism-specific biosystem; NGF-independant TRKA activation, organism-specific biosystem;
MIM	600456

Quick Inquiry

Case Study

Q & A

Customer Reviews

Here, researchers investigated the role of the neurotrophic tyrosine kinase receptor type 2 (NTRK2) gene in regulating the proliferation and invasion of human gastric cancer cells in vitro. The results showed that NTRK2 expression levels were abnormally elevated in gastric cancer cell lines compared to normal gastric cells. In MKN-28 and SNU-719 cells, downregulation of NTRK2 expression inhibited gastric cancer cell proliferation and invasion in vitro, while upregulation of NTRK2 expression promoted their proliferation and invasion. miR-22 was identified as an upstream negative regulator of NTRK2. In miR-22-upregulated MKN-28 and SNU-719 cells, overexpression of NTRK2 partially reversed the inhibitory effect of miR-22 on cancer cell proliferation and invasion. NTRK2 is an oncogene, and its expression level is negatively correlated with miR-22, jointly regulating the in vitro growth and development of gastric cancer cells.

Researchers investigated the effects of downregulating or upregulating NTRK2 on gastric cancer cell function. The results showed that compared to control gastric cancer cells, NTRK2 knockdown significantly reduced the proliferation rate of gastric cancer cells (Figure 1a). On the other hand, the cell proliferation rate was significantly increased in MKN-28 and SNU-719 cells overexpressing NTRK2 (Figure 1a). Furthermore, transfected gastric cancer cells were resuspended and tested using a Transwell invasion assay. The results showed that compared to control cells, NTRK2 knockdown significantly reduced the invasive ability of MKN-28 and SNU-719 cells (Figure 1b, c). Conversely, compared to control cells, NTRK2-overexpressing cells showed a significantly enhanced invasive capacity (Figure 1b, c). Therefore, these results indicate that NTRK2 is a functional regulator in gastric cancer, with its downregulation inhibiting cancer cell proliferation and invasion, while its upregulation promotes cancer cell proliferation and invasion.

Figure 1. NTRK2 downregulation or upregulation had functional roles in regulating GC proliferation and invasion. (Hu J, et al., 2016)

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *