Human NTRK2 Stable Cell Line - Ba/F3

Name: Human NTRK2 Stable Cell Line - Ba/F3
SKU: CSC-RO0412
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0412

Host Cell : Ba/F3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Cat. No.	CSC-RO0412
Description	This cell line is engineered to stably overexpress human neurotrophic receptor tyrosine kinase 2 (NTRK2, NM_006180.6). Expression of human NTRK2 in this cell line has been analyzed by qPCR.
Background	NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in NTRK2 gene have been associated with obesity and mood disorders.
Target Gene	NTRK2
Gene Species	Homo sapiens (Human)
Host Cell	Ba/F3
Host Cell Species	Mus musculus (Mouse)
Applications	Drug screening and biological assays
Biosafety Level	2
Size	>1x10^6 frozen cells/vial, 1 mL
Stability	Stable in culture over a minimum of 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Growth Conditions	37 °C, 5% CO₂
Storage	Liquid nitrogen
Shipping	Dry ice

Thawing & Subculturing Instructions	1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL.
Freeze Medium	Frozen with 70% medium, 20% FBS, 10% DMSO
Growth Properties	Suspension, round
Freezing Instructions	Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NTRK2 neurotrophic tyrosine kinase, receptor, type 2 [ Homo sapiens ]
Gene Symbol	NTRK2
Synonyms	TRKB; trk-B; GP145-TrkB
Gene Description	neurotrophic tyrosine kinase, receptor, type 2
Gene ID	4915
Uni Prot ID	Q16620
m RNA Refseq	NM_006180.3
Protein Refseq	NP_006171.2
Chromosome Location	9q22.1
Function	ATP binding; brain-derived neurotrophic factor binding; brain-derived neurotrophic factor-activated receptor activity; neurotrophin binding; neurotrophin binding; protein homodimerization activity;
Pathway	Activation of TRKA receptors, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; NGF signalling via TRKA from the plasma membrane, organism-specific biosystem; NGF-independant TRKA activation, organism-specific biosystem;
MIM	600456

CSC-RO0412-Human NTRK2 Stable Cell Line - Ba/F3-Datasheet

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