Panoply™ Human NRP1 Over-expressing Stable Cell Line

Name: Panoply™ Human NRP1 Over-expressing Stable Cell Line
SKU: CSC-SC010632
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC010632

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC010632
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	NRP1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NRP1 neuropilin 1 [ Homo sapiens ]
Gene Symbol	NRP1
Synonyms	NRP1; neuropilin 1; neuropilin-1; CD304; NRP; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; NP1; BDCA4; DKFZp781F1414; DKFZp686A03134;
Gene ID	8829
Uni Prot ID	O14786
m RNA Refseq	BC007737
Chromosome Location	10p12
Function	coreceptor activity; cytokine binding; growth factor binding; growth factor binding; heparin binding; metal ion binding; receptor activity; semaphorin receptor activity; vascular endothelial growth factor-activated receptor activity;
Pathway	Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; CHL1 interactions, organism-specific biosystem; CRMPs in Sema3A signaling, organism-specific biosystem; Developmental Biology, organism-specific biosystem; HTLV-I infection, organism-specific biosystem;
MIM	602069

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Neuropilin-1 (NRP-1), a multifunctional co-receptor, participates in cancer cell proliferation and metastasis by interacting with multiple signaling pathways. However, research on the precise function and prognostic analysis of NRP1 in intrahepatic cholangiocarcinoma (ICC) is scarce. Here, researchers explored the correlation between NRP1 and clinicopathological features of ICC and its impact on ICC cell line function. Results showed that NRP1 protein and mRNA levels were significantly elevated in ICC tissues compared to paired adjacent normal tissues. High NRP1 expression in ICC tissues was associated with poor prognosis. NRP1 expression levels are expected to be an independent prognostic factor for overall survival and cumulative tumor recurrence, and are closely related to tumor number. In vitro experiments showed that knockdown of NRP1 inhibited the proliferation and migration of RBE cells, while overexpression of NRP1 in 9810 cells promoted their proliferation and migration. Furthermore, NRP1 may promote ICC cell proliferation and migration through the FAK/PI3K/AKT pathway. As an oncogene, NRP1 may become a potential target for ICC treatment and a prognostic biomarker.

To further validate the role of NRP1 in ICC cell lines, researchers overexpressed NRP1 in the 9810 cell line, which had the lowest NRP1 expression level. In Figure 1, normal control cells were defined as the 9810-NC group, and the NRP1-overexpressing cell line was defined as the 9810-OE-NRP1 group. After overexpression, the expression of NRP1 mRNA in the 9810-OE-NRP1 group was significantly increased (Figure 1A). Figure 1C shows the results of transfection efficiency validation at the protein level. Figure 1B shows the results of the CCK-8 assay, Figures 1D and E show the results of the Transwell assay, and Figures 1F and G show the results of the wound healing assay. The results indicate that NRP1 overexpression promotes the proliferation, migration, and invasion of 9810 cells.

Figure 1. Cellular proliferation and migration comparison after NRP1 overexpression in the 9180 cell line. (Wu Y N, et al., 2020)

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