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Human NRP1 Stable Cell Line - HEK293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO0546

Host Cell :   HEK293T Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO0546
Description This cell line is derived from HEK293T and is engineered to stably overexpress Human NRP1.
Target Gene NRP1
Gene Species Homo sapiens (Human)
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Disease research

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name NRP1 neuropilin 1 [ Homo sapiens ]
Gene Symbol NRP1
Synonyms NRP1; neuropilin 1; neuropilin-1; CD304; NRP; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; NP1; BDCA4; DKFZp781F1414; DKFZp686A03134;
Gene ID 8829
Uni Prot ID O14786
m RNA Refseq BC007737
Chromosome Location 10p12
Function coreceptor activity; cytokine binding; growth factor binding; growth factor binding; heparin binding; metal ion binding; receptor activity; semaphorin receptor activity; vascular endothelial growth factor-activated receptor activity;
Pathway Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; CHL1 interactions, organism-specific biosystem; CRMPs in Sema3A signaling, organism-specific biosystem; Developmental Biology, organism-specific biosystem; HTLV-I infection, organism-specific biosystem;
MIM 602069
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