Panoply™ Human CD96 Over-expressing Stable Cell Line

Name: Panoply™ Human CD96 Over-expressing Stable Cell Line
SKU: CSC-SC002783
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC002783

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC002783
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	CD96
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD96 CD96 molecule [ Homo sapiens ]
Gene Symbol	CD96
Synonyms	TACTILE
Gene Description	CD96 molecule
Gene ID	10225
Uni Prot ID	P40200
m RNA Refseq	NM_198196.2
Protein Refseq	NP_937839.1
Chromosome Location	3q13.13-q13.2
Pathway	Adaptive Immune System, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem;
MIM	606037

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The poliovirus receptor (PVR) and its receptor system, including TIGIT, CD226, and CD96, play a key role in regulating tumor immune escape. When CD96 binds to PVR on tumor cells, it inhibits the function of T cells and NK cells, thereby promoting tumor immune escape. Therefore, screening immune checkpoint inhibitors (ICIs) targeting the CD96/PVR pathway will provide promising candidate drugs for tumor immunotherapy. Here, researchers used MOE software to virtually screen small molecules from the FDA-approved drug library. The results showed that epinastine has a high affinity for CD96, thereby effectively blocking the interaction between CD96 and PVR. In vitro co-culture experiments further showed that epinastine can effectively restore the ability of Jurkat cells to secrete IL-2. In the MC38 tumor model, epinastine significantly enhanced the infiltration of T cells and NK cells into the tumor site and increased their secretion of IFN-γ, thereby effectively inhibiting tumor growth.

It has been reported that CD96 is located on immune cells and can exert an immunosuppressive effect after binding to its ligand PVR. Epinastine can bind to CD96 and block CD96/PVR binding at the protein level. To detect whether epinastine can bind to CD96 on the cell surface, the researchers performed a cellular thermal shift assay (CETSA), and the results showed that epinastine treatment significantly increased the thermal stability of CD96 (Figure 1A, B), indicating that epinastine targets CD96 on the cell surface. Therefore, they also conducted a co-culture experiment to explore the effect of epinastine on the recovery of immune cell function. First, a Jurkat-hCD96 cell line overexpressing human CD96 was established (Figure 1C). Subsequently, Jurkat-hCD96 was co-cultured with CHO-K1-hPVR cells. Epinastine significantly enhanced the IL-2 secretion of Jurkat-hCD96 cells, and its effect was comparable to that of anti-hCD96 antibodies (Figure 1D, E). The results highlight that epinastine can target CD96 on immune cells, thereby inhibiting the binding of CD96 to PVR and alleviating its immunosuppressive effects on immune cells. In summary, these results show that epinastine can target CD96 on immune cells, thereby inhibiting CD96/PVR binding and alleviating its immunosuppressive effects on immune cells.

Figure 1. Effects of Epinastine on immune cell activity in vitro. (Zhang X, et al., 2025)

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