Monkey CD96 Stable Cell Line-HEK293T

Name: Monkey CD96 Stable Cell Line-HEK293T
SKU: CSC-RO1023
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1023

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO1023
Description	HEK293-CD96 cell line is engineered to stably overexpress monkey CD96.
Target Gene	CD96
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

How is the Monkey CD96 Stable Cell Line-HEK293 constructed?

A: The Monkey CD96 Stable Cell Line-HEK293 is constructed by introducing the CD96 gene into HEK293 cells using viral vectors or transfection techniques, allowing the cells to stably express the CD96 protein.

What is the role of Monkey CD96 Stable Cell Line-HEK293 in studying cross-species disease transmission?

A: This cell line can be used to study the role of CD96 in the process of viral or bacterial cross-species transmission, which helps reveal the mechanisms of disease transmission and develop novel prevention and control strategies.

How can the functionality of Monkey CD96 Stable Cell Line-HEK293 be evaluated?

A: The functionality of this cell line can be evaluated by detecting the expression level of CD96 protein, its localization on the cell surface, and its interactions with other proteins.

What are the applications of Monkey CD96 Stable Cell Line-HEK293 in drug screening?

A: This cell line can be used to screen and evaluate drugs targeting CD96-related pathways, such as assessing the potential efficacy and safety of drugs by detecting changes in CD96 expression or function after drug treatment.

How can the reproducibility of experimental results be ensured when using Monkey CD96 Stable Cell Line-HEK293?

A: To ensure the reproducibility of experimental results, it is necessary to strictly control experimental conditions, such as cell culture conditions, experimental operation steps, and reagent quality. At the same time, multiple independent experiments should be conducted to verify the stability of the results.

How can Monkey CD96 Stable Cell Line-HEK293 cells be stored to maintain their stability?

A: The cells can be stored in liquid nitrogen to maintain their stability. Before freezing, cells can be suspended in culture medium containing an appropriate concentration of cryoprotectant (such as DMSO) and gradually cooled in a programmable freezer. When thawing, quickly transfer the cells to a 37°C water bath and then add pre-warmed culture medium to reduce cell damage.

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