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Human CD96 Stable Cell Line-HEK293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO1056

Host Cell :   HEK293T Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO1056
Description HEK293-CD96 cell line is engineered to stably overexpress human CD96.
Target Gene CD96
Gene Species Homo sapiens (Human)
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Applications

1. Studying the interactions between immune cells and cancer cells

2. Studying the mechanisms of resistance to immune checkpoint blockade

3. High-throughput screening

4. Drug target validation

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name CD96 CD96 molecule [ Homo sapiens ]
Gene Symbol CD96
Synonyms TACTILE
Gene Description CD96 molecule
Gene ID 10225
Uni Prot ID P40200
m RNA Refseq NM_198196.2
Protein Refseq NP_937839.1
Chromosome Location 3q13.13-q13.2
Pathway Adaptive Immune System, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem;
MIM 606037
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Q & A

Customer Reviews

Customer Q&As
How was the Human CD96 Stable Cell Line-HEK293 established?

A: The cell line was established by stably transfecting the human CD96 gene into HEK293 cells, aiming to enable stable expression of the CD96 protein in the cells.

What is the role of the Human CD96 Stable Cell Line-HEK293 in tumor immunotherapy research?

A: This cell line can be used to study the role of CD96 in tumor immune responses, especially in investigating its effects on the activation of immune cells and tumor cell escape mechanisms.

What should be considered when using the Human CD96 Stable Cell Line-HEK293 for drug screening?

A: It is important to set the screening conditions carefully to ensure accurate assessment of the drug's impact on CD96 protein activity, while avoiding cytotoxic effects on the cells.

What is the importance of the Human CD96 Stable Cell Line-HEK293 in immune checkpoint research?

A: Since CD96 is related to immune checkpoints, this cell line is very important for studying the mechanisms and effects of immune checkpoint inhibitors.

What are the advantages of this cell line in simulating the human immune system?

A: It can stably express human CD96, effectively simulating the role of CD96 in the human immune system, which is helpful for studying immune-related diseases.

In what types of disease models is the Human CD96 Stable Cell Line-HEK293 particularly important?

A: It is particularly important in studying disease models related to tumor immune escape, autoimmune diseases, etc.

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