Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO1056
Host Cell : HEK293T Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO1056 |
| Description | HEK293-CD96 cell line is engineered to stably overexpress human CD96. |
| Target Gene | CD96 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | CD96 CD96 molecule [ Homo sapiens ] |
| Gene Symbol | CD96 |
| Synonyms | TACTILE |
| Gene Description | CD96 molecule |
| Gene ID | 10225 |
| Uni Prot ID | P40200 |
| m RNA Refseq | NM_198196.2 |
| Protein Refseq | NP_937839.1 |
| Chromosome Location | 3q13.13-q13.2 |
| Pathway | Adaptive Immune System, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem; |
| MIM | 606037 |
A: The cell line was established by stably transfecting the human CD96 gene into HEK293 cells, aiming to enable stable expression of the CD96 protein in the cells.
A: This cell line can be used to study the role of CD96 in tumor immune responses, especially in investigating its effects on the activation of immune cells and tumor cell escape mechanisms.
A: It is important to set the screening conditions carefully to ensure accurate assessment of the drug's impact on CD96 protein activity, while avoiding cytotoxic effects on the cells.
A: Since CD96 is related to immune checkpoints, this cell line is very important for studying the mechanisms and effects of immune checkpoint inhibitors.
A: It can stably express human CD96, effectively simulating the role of CD96 in the human immune system, which is helpful for studying immune-related diseases.
A: It is particularly important in studying disease models related to tumor immune escape, autoimmune diseases, etc.
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