Panoply™ Human ACVR2A Knockdown Stable Cell Line

Name: Panoply™ Human ACVR2A Knockdown Stable Cell Line
SKU: CSC-DC000217
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC000217

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-DC000217
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	ACVR2A
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ACVR2A activin A receptor, type IIA [ Homo sapiens ]
Gene Symbol	ACVR2A
Synonyms	ACVR2; ACTRII
Gene Description	activin A receptor, type IIA
Gene ID	92
Uni Prot ID	P27037
m RNA Refseq	NM_001616.3
Protein Refseq	NP_001607.1
Chromosome Location	2q22.3
Function	ATP binding; PDZ domain binding; contributes_to activin binding; activin receptor activity, type II; contributes_to activin-activated receptor activity; coreceptor activity; growth factor binding; inhibin binding; metal ion binding; protein binding; contributes_to protein binding; protein self-association; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity; transforming growth factor beta-activated receptor activity; transmembrane receptor protein serine/threonine kinase activity;
Pathway	ALK1 signaling events, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Developmental Biology, organism-specific biosystem; Regulation of Signaling by NODAL, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by BMP, organism-specific biosystem;
MIM	102581

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Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor-β (TGF-β) signaling pathway, involved in regulating cell proliferation, migration, and apoptosis. Here, researchers examined the expression profile and biological function of ACVR2A in colon cancer. In the GSE39582 database, ACVR2A mRNA expression was identified as a prognostic factor by linear regression analysis. In a validation cohort of 15 patients with stage IV cancer, ACVR2A mRNA expression was significantly decreased in metastatic lesions and primary tumors compared with adjacent normal controls. In another tissue microarray (TMA) validation cohort of 193 cases, decreased ACVR2A protein expression was associated with advanced N stage and positive lymphovascular invasion. A strong correlation between low ACVR2A mRNA or protein expression and poor survival was also observed in both the GSE39582 database and the TMA validation cohort. Furthermore, in vitro studies demonstrated significantly increased cell migration in ACVR2A knockdown cells. These findings suggest that loss of ACVR2A plays an important role in cancer progression and distant metastasis and may serve as a prognostic marker for patients with colon cancer.

To evaluate the biological function of ACVR2A in colon cancer, the researchers generated stable ACVR2A knockdown RKO and HCT116 cell lines (Figures 1a and b). The results showed that ACVR2A knockdown did not affect colon cancer cell proliferation (Figure 1c). Next, they used Transwell assays to investigate whether ACVR2A knockdown affected colon cancer cell migration. The results showed that the number of migrating cells increased two- to three-fold in the ACVR2A knockdown cell lines compared to the control group (Figure 1d).

Figure 1. Silencing of ACVR2A expression promotes the migration of human colon cancer cells. (Zhuo C, et al., 2018)

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