MIR150-pCMV-MIR

Name: MIR150-pCMV-MIR
SKU: MIPM2031

For research use only. Not intended for any clinical use.

Cat.No.

MIPM2031

Description

Expression plasmid for mouse microRNA Mir150. All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA. Both mature and minor (star) miRNA can be over expressed from the construct.

Reporter

GFP

Size/Form

The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5 and 3 vector primers in separate vials.

Species

Mouse

Vector Type

plasmid

miRNA Information

Precursor Accession No.

MI0000172

Precursor Name

mmu-mir-150

Precursors sequence

CCCUGUCUCCCAACCCUUGUACCAGUGCUGUGCCU CAGACCCUGGUACAGGCCUGGGGGAUAGGG

Precursors Length

65 bp

Gene Description

Mus musculus miR-150 stem-loop

Gene Species

Mouse

Mature miRNA-1 Accession No.

MIMAT0000160

Mature miRNA-1 Name

mmu-miR-150-5p

Mature miRNA-1 sequence

UCUCCCAACCCUUGUACCAGUG

Mature miRNA-2 Accession No.

MIMAT0004535

Mature miRNA-2 Name

mmu-miR-150-3p

Mature miRNA-2 sequence

CUGGUACAGGCCUGGGGGAUAG

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