Cat.No. : CSC-RO0047 Host Cell: CHO-K1
| Cat. No. | CSC-RO0047 |
| Description | This cell line is a stably transfected cell line which expresses Human TNFSF4 without any tag. |
| Background | This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. |
| Gene | TNFSF4 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | TNFSF4 tumor necrosis factor superfamily member 4 [ Homo sapiens (human) ] |
| Gene Symbol | TNFSF4 |
| Synonyms | GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B |
| Gene ID | 7292 |
| UniProt ID | P23510 |
| Chromosome Location | 1q25.1 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: CHO cells, including the Human TNFSF4 Stable Cell Line, can exhibit various responses to stressors. Oxidative stress may lead to increased production of reactive oxygen species (ROS), which can be mitigated by antioxidants in the culture medium. Temperature shifts can affect cell growth rates and protein folding, with cells typically adapting to new conditions over time. Monitoring and adjusting culture parameters accordingly is essential for maintaining cell viability and productivity.
A: Induction of TNFSF4 expression in the Human TNFSF4 Stable Cell Line-CHO is typically achieved through the addition of chemical inducers, such as sodium butyrate or tetracycline, to the culture medium. These inducers bind to specific receptors or pathways within the cells, leading to the activation of the TNFSF4 gene and subsequent protein production.
A: Ensuring the quality and purity of TNFSF4 protein involves several steps. After protein expression, cells are lysed, and the protein is purified using techniques such as affinity chromatography, ion exchange, or size exclusion chromatography. Quality control measures include SDS-PAGE for protein size confirmation, Western blotting for specific antigen recognition, and mass spectrometry for protein identity and purity assessment.
A: TNFSF4, also known as OX40L, is a protein with potential therapeutic applications in the field of immunology. It is involved in T-cell activation and has been studied for its role in enhancing immune responses in cancer immunotherapy. The protein produced by the Human TNFSF4 Stable Cell Line-CHO could be used for the development of biologic drugs aimed at treating various diseases, including cancer and autoimmune disorders.
A: CHO cells are known for their ability to perform complex glycosylation, which can affect protein function and stability. The Human TNFSF4 Stable Cell Line-CHO may exhibit unique glycosylation patterns compared to other CHO cell lines due to genetic modifications and the specific culture conditions. These differences can influence the protein's efficacy and immunogenicity, making it crucial to characterize the glycosylation profile for therapeutic applications.
A: Strategies to improve productivity include genetic engineering to enhance gene expression, optimizing culture conditions for cell growth and protein production, and employing advanced bioprocess technologies like fed-batch or perfusion systems. Additionally, the use of proteomic and metabolomic analyses can provide insights into cellular stress and metabolic pathways, guiding targeted improvements in the production process.
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