Cat.No. : CSC-RO0557 Host Cell: HEK293T
| Cat. No. | CSC-RO0557 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Cyno TNFSF4. |
| Gene | TNFSF4 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The role of the Monkey TNFSF4 Stable Cell Line - HEK293T in studying the TNFSF4 signaling pathway primarily lies in its stable expression of TNFSF4, which allows researchers to delve into the functions of TNFSF4 in cell signaling, immune regulation, and inflammatory responses. Through this cell line, scientists can simulate the interaction between TNFSF4 and its receptor TNFRSF4, studying its role in immune cell activation, proliferation, and differentiation, as well as its potential applications in autoimmune diseases and cancer therapy.
A: The Monkey TNFSF4 Stable Cell Line - HEK293T cell line can be utilized for drug screening by employing high-throughput screening techniques to identify small molecule compounds or biotherapeutics that modulate the expression or signaling pathway of TNFSF4. These screened candidates can then be further evaluated at the cellular level for their impact on the TNFSF4 signaling pathway, providing potential therapeutic strategies for related diseases.
A: The value of the Monkey TNFSF4 Stable Cell Line - HEK293T cell line in studying the interaction between TNFSF4 and immune cells lies in providing a stable platform for investigating how TNFSF4 affects the activation status and function of immune cells. This cell line can be used to simulate the role of TNFSF4 in immune responses, particularly in the activation and differentiation processes of T cells and B cells, which is crucial for understanding the role of TNFSF4 in immune regulation.
A: The potential application of the Monkey TNFSF4 Stable Cell Line - HEK293T cell line in gene editing research is vast. Due to the efficient gene editing capability of the HEK293T cell line, this cell line can be used to study how specific mutations in the TNFSF4 gene affect its function and the role of these mutations in disease development. Additionally, this cell line can be used to develop and optimize gene editing tools, such as the CRISPR/Cas9 system, to improve their editing efficiency and specificity on the TNFSF4 gene.
A: The Monkey TNFSF4 Stable Cell Line - HEK293T cell line can be used to simulate the role of TNFSF4 in the tumor microenvironment, particularly in the interactions between tumor cells and immune cells. This cell line can be used to study how TNFSF4 affects the growth, invasion, and metastasis of tumor cells, as well as how it modulates the function of immune cells in the tumor microenvironment, which is significant for developing new cancer treatment strategies.
A: The potential applications of the Monkey TNFSF4 Stable Cell Line - HEK293T cell line in the development of TNFSF4-related biotherapeutics include using it for evaluating the biological activity, stability, and safety of biotherapeutics. This cell line can be used for high-throughput screening studies to identify small molecule drugs or biotherapeutics that modulate the function of TNFSF4. Additionally, this cell line can be used to assess the efficacy and potential side effects of biotherapeutics in simulating human immune responses.
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