Panoply™ Human TNFSF4 Over-expressing Stable Cell Line

Name: Panoply™ Human TNFSF4 Over-expressing Stable Cell Line
SKU: CSC-SC016380
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016380

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC016380
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TNFSF4
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFSF4 tumor necrosis factor (ligand) superfamily, member 4 [ Homo sapiens ]
Gene Symbol	TNFSF4
Synonyms	GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L
Gene ID	7292
Uni Prot ID	P23510
m RNA Refseq	NM_003326.3
Protein Refseq	NP_003317.1
Chromosome Location	1q25
Function	cytokine activity; receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor superfamily binding;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; TSLP Signaling Pathway, organism-specific biosystem;
MIM	603594

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As a member of the tumor necrosis factor (TNF) superfamily, tumor necrosis factor superfamily member 4 (TNFSF4) is expressed on antigen-presenting cells and activates T cells by binding to its receptor TNFRSF4. However, the tumorigenicity of TNFSF4 has not been studied in a pan-cancer population. Here, researchers performed a comprehensive bioinformatics analysis of the pan-cancer population to explore the mechanism by which TNFSF4 regulates tumorigenesis. Pan-cancer population analysis showed that TNFSF4 was upregulated in multiple tumors. There was also a significant correlation between TNFSF4 expression and single-cell data in multiple cancer types. TNFSF4 expression was associated with the expression of immune checkpoint genes and may affect the sensitivity of multiple drugs. In vitro and in vivo experiments showed that TNFSF4 can promote the occurrence and development of hepatocellular carcinoma (HCC). TNFSF4 is upregulated in multiple cancer types and promotes the occurrence and development of cancer through multiple mechanisms such as regulating tumor infiltration of immune cells. These studies indicate that TNFSF4 is a promising prognostic and immunotherapy biomarker in certain malignancies.

The researchers detected the expression level of TNFSF4 in tumor tissues of 11 HCC patients by qRT-PCR. The results showed that the mRNA level of TNFSF4 in HCC tissues was significantly higher than that in the corresponding normal liver tissues, which was consistent with the results of the TCGA database (Figure 1A). Subsequently, TNFSF4 was overexpressed in Hepa1-6 cells using a plasmid that specifically expressed TNFSF4. Immunostaining results showed that the TNFSF4 overexpression plasmid successfully overexpressed TNFSF4 (Figure 10). The results of the scratch repair experiment, Transwell experiment and clone formation experiment showed that TNFSF4 overexpression promoted the migration, invasion and proliferation of Hepa1-6 cells (Figure 1C-I). In addition, tumor xenograft experiments showed that TNFSF4 overexpression promoted the in vivo growth of Hepa1-6 cells. These results indicate that TNFSF4 promotes the proliferation, invasion and migration of Hepa1-6 cells in vivo and in vitro.

Figure 1. The biological functions of TNFSF4 in HCC. (Deng Z, et al., 2025)

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