Cat.No. : AD00257Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00257Z |
| Target Gene | SLC2A7 |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | SLC2A7 |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Gene Name | SLC2A7 solute carrier family 2 (facilitated glucose transporter), member 7 [ Homo sapiens ] |
| Gene Symbol | SLC2A7 |
| Synonyms | GLUT7 |
| Gene ID | 155184 |
| UniProt ID | Q6PXP3 |
| mRNA Refseq | NM_207420.2 |
| Protein Refseq | NP_997303.2 |
| Chromosome Location | 1p36.2 |
| Function | substrate-specific transmembrane transporter activity; |
| Pathway | Class II GLUTs, organism-specific biosystem; Facilitative Na+-independent glucose transporters, organism-specific biosystem; SLC-mediated transmembrane transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem; Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds, organism-specific biosystem; |
| MIM | 610371 |
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide. Although the mechanisms of HCC progression are not well understood, recent studies have suggested the potential contribution of the uric acid transporter SLC2A9 to tumor suppression. Here, researchers found that SLC2A9 expression was reduced in human HCC tissues and cell lines. Moreover, overexpression of SLC2A9 inhibited HCC cell proliferation. SLC2A9 induced apoptosis in HCC cells by inhibiting the expression of caspase 3. The study also showed that upregulation of SLC2A9 reduced the accumulation of intracellular reactive oxygen species (ROS). In addition, SLC2A9 increased the mRNA and protein expression of the tumor suppressor p53 in HCC cells. Probenecid inhibited SLC2A9-mediated uric acid transport, promoted HCC cell proliferation, inhibited apoptosis, induced intracellular ROS, and reduced the expression of p53 in HCC cells. Therefore, this study suggests that SLC2A9 may be a novel tumor suppressor gene and a potential therapeutic target for HCC.
Here, the researchers conducted flow cytometry experiments to investigate the effect of SLC2A9 on cell apoptosis. The results showed that in HepG2, apoptosis was observed after SLC2A9 adenovirus (Adv-SCL2A9) transfection, while apoptosis was reduced in the probenecid group (Figure 1). In addition, the proportion of apoptotic cells in the Adv-SLC2A9 group (44%) increased compared with the negative control group (20%), while the proportion of apoptotic cells in the probenecid group (10%) decreased compared with the negative control group (20%) (Figure 1B). Caspase 3 is closely related to cell apoptosis. Therefore, Western blotting was also performed to detect the mRNA and protein expression of caspase 3. The results showed that SLC2A9 increased the mRNA and protein expression of caspase 3 (Figure 1C and D). Probenecid decreased the mRNA and protein expression of SLC2A9. These data indicate that SLC2A9 induces apoptosis in HCC HepG2 cells.
Figure 1. SLC2A9 induces cell apoptosis in HepG2 cells. (Han X, et al., 2019)
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These adenoviral particles efficiently delivered the SLC2A9 gene into our target cells. Minimal toxicity and consistent results—great for functional studies!
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