Human OPRK1-tGFP Stable Cell Line-U2OS

Name: Human OPRK1-tGFP Stable Cell Line-U2OS
SKU: CSC-RG0034
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0034

Host Cell : U2OS Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0034
Background	kappa-opioid receptor is one of five related receptors that bind opium-like compounds in the brain and are responsible for mediating the effects of these compounds. These effects include altering the perception of pain, consciousness, motor control, and mood. Opioid receptors mediate the analgesic action and addictive properties of opioid drugs. The kappa-opioid receptor may provide a natural addiction control mechanism, and consequently selective agonists of this receptor may have therapeutic potential in the treatment of addiction.
Target Gene	OPRK1
Gene Species	Homo sapiens (Human)
Abbr	U2OS-HuOPRK1-tGFP
Alias	OPRK1, KOR, KOR-1, K-OR-1, OPRK
Host Cell	U2OS
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	OPRK1 opioid receptor, kappa 1 [ Homo sapiens ]
Gene Symbol	OPRK1
Synonyms	KOR; OPRK
Gene Description	opioid receptor, kappa 1
GeneID	4986
Uni ProtID	P41145
mRNA Refseq	NM_000912.3
Protein Refseq	NP_000903.2
Chromosome Location	8q11.2
Function	dynorphin receptor activity; protein binding;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem;
MIM	165196

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How does the Human OPRK1-tGFP Stable Cell Line-U2OS facilitate the investigation of opioid receptor trafficking?

A: The U2OS cell line stably expressing OPRK1-tGFP provides a platform to monitor receptor trafficking in real-time. By observing the movement and clustering of the fluorescently tagged receptor, researchers can gain insights into the mechanisms of receptor internalization, recycling, and degradation, which are critical aspects of GPCR function.

What are the technical considerations for maintaining the Human OPRK1-tGFP Stable Cell Line-U2OS in culture?

A: To maintain the stability of the OPRK1-tGFP expression, cells should be cultured under optimal conditions, including appropriate temperature, CO2 levels, and medium composition. Regular passage and selection with antibiotics or other selection agents may be necessary to ensure that only cells with the stable OPRK1-tGFP expression are propagated.

How can the Human OPRK1-tGFP Stable Cell Line-U2OS be used to screen for compounds that modulate OPRK1 activity?

A: The cell line can be utilized in high-throughput screening assays to identify compounds that alter the fluorescence pattern of OPRK1-tGFP, indicating changes in receptor activity. This can lead to the discovery of new drugs that target the mu-opioid receptor for therapeutic purposes.

What is the role of the Human OPRK1-tGFP Stable Cell Line-U2OS in the study of opioid-induced tolerance and dependence?

A: This cell line enables researchers to examine the cellular and molecular changes associated with opioid tolerance and dependence. By tracking the behavior of the fluorescently labeled OPRK1 receptor, scientists can investigate how chronic exposure to opioids affects receptor function and cellular signaling.

How does the Human OPRK1-tGFP Stable Cell Line-U2OS contribute to the understanding of GPCR dimerization?

A: The cell line can be used to study the potential dimerization of OPRK1 with other GPCRs, as the tGFP tag can be used to visualize and quantify interactions between different receptors. This can provide insights into the complex mechanisms of GPCR signaling and regulation.

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