Cat.No. : CSC-RG0034 Host Cell: U2OS
| Cat. No. | CSC-RG0034 |
| Background | kappa-opioid receptor is one of five related receptors that bind opium-like compounds in the brain and are responsible for mediating the effects of these compounds. These effects include altering the perception of pain, consciousness, motor control, and mood. Opioid receptors mediate the analgesic action and addictive properties of opioid drugs. The kappa-opioid receptor may provide a natural addiction control mechanism, and consequently selective agonists of this receptor may have therapeutic potential in the treatment of addiction. |
| Gene | OPRK1 |
| Gene Species | Homo sapiens (Human) |
| Alias | OPRK1, KOR, KOR-1, K-OR-1, OPRK |
| Host Cell | U2OS |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The U2OS cell line stably expressing OPRK1-tGFP provides a platform to monitor receptor trafficking in real-time. By observing the movement and clustering of the fluorescently tagged receptor, researchers can gain insights into the mechanisms of receptor internalization, recycling, and degradation, which are critical aspects of GPCR function.
A: To maintain the stability of the OPRK1-tGFP expression, cells should be cultured under optimal conditions, including appropriate temperature, CO2 levels, and medium composition. Regular passage and selection with antibiotics or other selection agents may be necessary to ensure that only cells with the stable OPRK1-tGFP expression are propagated.
A: The cell line can be utilized in high-throughput screening assays to identify compounds that alter the fluorescence pattern of OPRK1-tGFP, indicating changes in receptor activity. This can lead to the discovery of new drugs that target the mu-opioid receptor for therapeutic purposes.
A: This cell line enables researchers to examine the cellular and molecular changes associated with opioid tolerance and dependence. By tracking the behavior of the fluorescently labeled OPRK1 receptor, scientists can investigate how chronic exposure to opioids affects receptor function and cellular signaling.
A: The cell line can be used to study the potential dimerization of OPRK1 with other GPCRs, as the tGFP tag can be used to visualize and quantify interactions between different receptors. This can provide insights into the complex mechanisms of GPCR signaling and regulation.
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