Human KCNH2 Stable Cell Line-HEK293

Name: Human KCNH2 Stable Cell Line-HEK293
SKU: CSC-RI0135
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0135

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0135
Description	This cell line is engineered to overexpress human KCNH2.
Background	The human KCNH2 gene encodes the pore-forming subunit of Kv11.1, the voltage-gated hERG potassium channel. HERG channels are upregulated in some types of cancer and may serve as a tumor marker. KCNH2 mutations cause inherited forms of cardiac disorders including both long QT (loss-of-function) and short QT (gain-of-function) syndromes. Inhibition of HERG channels by pharmaceuticals is the primary cause of acquired long QT syndrome and drug-induced torsade de pointe. Therefore, HERG is an anti-target in cardiac risk assessment.
Target Gene	KCNH2
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNH2
Alias	KCNH2, LQT2, erg1, HERG, Kv11.1, ERG-1, H-ERG, hERG-1, ERG1, SQT1, HERG1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNH2 potassium voltage-gated channel, subfamily H (eag-related), member 2 [ Homo sapiens ]
Gene Symbol	KCNH2
Synonyms	ERG1; HERG; LQT2; SQT1; HERG1; Kv11.1
GeneID	3757
Uni ProtID	Q12809
mRNA Refseq	NM_000238.3
Protein Refseq	NP_000229.1
Chromosome Location	7q36.1
Function	delayed rectifier potassium channel activity; inward rectifier potassium channel activity; protein binding; two-component sensor activity; voltage-gated potassium channel activity;
Pathway	Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; SIDS Susceptibility Pathways, organism-specific biosystem; Voltage gated Potassium channels, organism-specific biosystem;
MIM	152427

Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *