Cat.No. : CSC-RI0121 Host Cell: HEK293
| Cat. No. | CSC-RI0121 |
| Description | This cell line is engineered to overexpress human KCNA1. |
| Background | The human KCNA1 gene encodes the pore-forming subunit of Kv1.1, a voltage-gated potassium channel. Mutations in KCNA1 cause several congenital disorders including episodic ataxia type 1, neuromyotonia, and autosomal dominant hypomagnesia. Kv1.1 channels expressed in neurons are therapeutic targets in multiple sclerosis, stroke and seizure. |
| Gene | KCNA1 |
| Gene Species | Homo sapiens (Human) |
| Alias | KCNA1, AEMK, HUK1, Kv1.1, MBK1, RBK1, EA1, MK1, HBK1, KV1.1, MGC126782, MGC138385 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The role of the KCNA1 channel in neurological diseases, such as epilepsy and neurodegenerative diseases, should be considered, especially in terms of drug targets and pathological mechanisms.
A: This cell line has high sensitivity to drugs affecting KCNA1 channel activity, making it suitable for screening potential anti-arrhythmic and neuroprotective drugs.
A: The cell line was established by using liposome-mediated transfection to stably incorporate the human KCNA1 gene (potassium channel subfamily A member 1) into the genome of HEK293 cells.
A: It can be used to study the function of KCNA1 in muscle cells, particularly in exploring its role in muscle diseases like periodic paralysis.
A: Due to stable expression of a specific potassium channel subtype, this cell line can provide detailed information on the biological characteristics and pharmacological responses of KCNA1 channels.
A: Given the critical role of KCNA1 in regulating the electrophysiological properties of cardiac cells, this cell line is particularly suitable for studying the molecular mechanisms related to arrhythmias.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
