Human KCNA1 Stable Cell Line-HEK293

Name: Human KCNA1 Stable Cell Line-HEK293
SKU: CSC-RI0121
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0121

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0121
Description	This cell line is engineered to overexpress human KCNA1.
Background	The human KCNA1 gene encodes the pore-forming subunit of Kv1.1, a voltage-gated potassium channel. Mutations in KCNA1 cause several congenital disorders including episodic ataxia type 1, neuromyotonia, and autosomal dominant hypomagnesia. Kv1.1 channels expressed in neurons are therapeutic targets in multiple sclerosis, stroke and seizure.
Target Gene	KCNA1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNA1
Alias	KCNA1, AEMK, HUK1, Kv1.1, MBK1, RBK1, EA1, MK1, HBK1, KV1.1, MGC126782, MGC138385
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNA1 potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia with myokymia) [ Homo sapiens ]
Gene Symbol	KCNA1
Synonyms	EA1; MK1; AEMK; HBK1; HUK1; MBK1; RBK1; KV1.1
Gene Description	potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia with myokymia)
GeneID	3736
Uni ProtID	Q09470
mRNA Refseq	NM_000217.2
Protein Refseq	NP_000208.2
Chromosome Location	12p13.32
Function	delayed rectifier potassium channel activity; potassium channel activity; potassium ion transmembrane transporter activity;
Pathway	Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Voltage gated Potassium channels, organism-specific biosystem;
MIM	176260

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Q & A

Customer Reviews

Customer Q&As

What should be considered when using this cell line for neurological disease research?

A: The role of the KCNA1 channel in neurological diseases, such as epilepsy and neurodegenerative diseases, should be considered, especially in terms of drug targets and pathological mechanisms.

How sensitive is the Human KCNA1 Stable Cell Line-HEK293 in drug screening experiments?

A: This cell line has high sensitivity to drugs affecting KCNA1 channel activity, making it suitable for screening potential anti-arrhythmic and neuroprotective drugs.

What specific transfection technique was used in the establishment of the Human KCNA1 Stable Cell Line-HEK293?

A: The cell line was established by using liposome-mediated transfection to stably incorporate the human KCNA1 gene (potassium channel subfamily A member 1) into the genome of HEK293 cells.

What are the applications of the Human KCNA1 Stable Cell Line-HEK293 in muscle disease model research?

A: It can be used to study the function of KCNA1 in muscle cells, particularly in exploring its role in muscle diseases like periodic paralysis.

What unique insights can the Human KCNA1 Stable Cell Line-HEK293 provide in ion channel research?

A: Due to stable expression of a specific potassium channel subtype, this cell line can provide detailed information on the biological characteristics and pharmacological responses of KCNA1 channels.

What are the unique advantages of using the Human KCNA1 Stable Cell Line-HEK293 in cardiac research?

A: Given the critical role of KCNA1 in regulating the electrophysiological properties of cardiac cells, this cell line is particularly suitable for studying the molecular mechanisms related to arrhythmias.

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