Cat.No. : CSC-RI0026 Host Cell: CHO-K1
| Cat. No. | CSC-RI0026 |
| Description | This cell line is engineered to overexpress human KCNA1. |
| Background | Kv1.1 is a voltage-gated, delayed rectifier, potassium channel. It is expressed in the brain, heart, retina, skeletal muscle and pancreatic islets and is primarily involved with maintaining the resting membrane potential and modulating electrical excitability in neurons and muscle cells. Mutations of this ion channel have been linked to episodic ataxia, an autosomal dominant human disorder that produces persistent myokymia (successive, involuntary, small muscle contractions or tics that affect a bundle of muscles). |
| Gene | KCNA1 |
| Gene Species | Homo sapiens (Human) |
| Alias | KCNA1, AEMK, HUK1, Kv1.1, MBK1, RBK1, EA1, MK1, HBK1, KV1.1, MGC126782, MGC138385 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The cell line was established by stably transfecting the human KCNA1 gene (potassium channel subfamily A member 1) into CHO (Chinese hamster ovary) cells.
A: It is mainly used to study the role of KCNA1 in the regulation of neuronal excitability and neural signal transduction.
A: It is suitable for screening drugs that affect the function of the KCNA1 channel, especially for research on treatments for neurodegenerative diseases and epilepsy.
A: It can be used to study the role of KCNA1 in cardiac cells, especially in the mechanisms of cardiac arrhythmias.
A: It has significant value in studies of disease models for neurological disorders such as epilepsy, motor neuron diseases, and cardiac arrhythmias.
A: By regularly performing genotypic and phenotypic testing to ensure the consistency of KCNA1 expression and stability of cell characteristics.
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