Human HCN4 Stable Cell Line-CHO-K1

Name: Human HCN4 Stable Cell Line-CHO-K1
SKU: CSC-RI0014
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0014

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0014
Description	This cell line is engineered to overexpress human HCN4.
Target Gene	HCN4
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuHCN4
Alias	HCN4, SSS2
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HCN4 hyperpolarization activated cyclic nucleotide-gated potassium channel 4 [ Homo sapiens ]
Gene Symbol	HCN4
Synonyms	SSS2
GeneID	10021
Uni ProtID	Q9Y3Q4
mRNA Refseq	NM_005477.2
Protein Refseq	NP_005468.1
Chromosome Location	15q24.1
Function	cAMP binding; cation channel activity; intracellular cAMP activated cation channel activity; sodium channel activity; voltage-gated potassium channel activity;
Pathway	HCN channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	605206

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Customer Reviews

HCN4 is the major Hyperpolarization-activated Cyclic Nucleotide-gated ion channel in the mammalian heart. They are voltage-gated, cation channels, typically closed at resting and depolarized membrane potentials. The action of HCN4 is essential for the rhythmicity of the heart beat as well as controlling the strength of the contraction. HCN4 is expressed in the heart and brain, but significantly, not in the retina. The side effects of many anti-arrhythmic drugs include visual disturbances; a selective HCN4 blocker would potentially slow the heart rate without affecting vision. Safety screening, to detect undesirable action on HCN4 channels, is essential to assess the cardiac liability of new drug submissions.

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