Human HCN4 Stable Cell Line-CHO

Name: Human HCN4 Stable Cell Line-CHO
SKU: CSC-RI0014

Cat.No. : CSC-RI0014 Host Cell: CHO-K1

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RI0014
Description	This cell line is engineered to overexpress human HCN4.
Gene	HCN4
Gene Species	Homo sapiens (Human)
Alias	HCN4, SSS2
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Morphology	Epithelial-like
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

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HCN4 is the major Hyperpolarization-activated Cyclic Nucleotide-gated ion channel in the mammalian heart. They are voltage-gated, cation channels, typically closed at resting and depolarized membrane potentials. The action of HCN4 is essential for the rhythmicity of the heart beat as well as controlling the strength of the contraction. HCN4 is expressed in the heart and brain, but significantly, not in the retina. The side effects of many anti-arrhythmic drugs include visual disturbances; a selective HCN4 blocker would potentially slow the heart rate without affecting vision. Safety screening, to detect undesirable action on HCN4 channels, is essential to assess the cardiac liability of new drug submissions.

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