Human HCN4 Stable Cell Line-HEK293

Name: Human HCN4 Stable Cell Line-HEK293
SKU: CSC-RI0102
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0102

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0102
Description	This cell line is engineered to overexpress human HCN4.
Background	The human HCN4 gene encodes the pore-forming subunit of a hyperpolarization-activated, cyclic nucleotide-modulated cation channel. HCN4 channels are the predominant HCN isoform in the sinoatrial node and contribute to pacemaker current that controls rhythmic activity in the heart and brain. The HCN4 therapeutic potential includes treatment of cardiac arrhythmia and ischemic heart disease.
Target Gene	HCN4
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuHCN4
Alias	HCN4, SSS2
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HCN4 hyperpolarization activated cyclic nucleotide-gated potassium channel 4 [ Homo sapiens ]
Gene Symbol	HCN4
Synonyms	SSS2
GeneID	10021
Uni ProtID	Q9Y3Q4
mRNA Refseq	NM_005477.2
Protein Refseq	NP_005468.1
Chromosome Location	15q24.1
Function	cAMP binding; cation channel activity; intracellular cAMP activated cation channel activity; sodium channel activity; voltage-gated potassium channel activity;
Pathway	HCN channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	605206

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Q & A

Customer Reviews

Customer Q&As

How was the Human HCN4 Stable Cell Line-HEK293 established?

A: The cell line was established by stably transfecting the human HCN4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) gene into HEK293 cells.

What specific applications does the Human HCN4 Stable Cell Line-HEK293 have in cardiac physiology research?

A: It is primarily used to study the role of HCN4 in cardiac rhythm regulation and arrhythmias.

What are the applications of the Human HCN4 Stable Cell Line-HEK293 in neuroscience research?

A: It can be used to study the role of HCN4 in neural signal transduction and the regulation of neuronal excitability.

What are the advantages of using the Human HCN4 Stable Cell Line-HEK293 in drug screening?

A: This cell line is suitable for screening drugs that affect HCN4 channel activity, important for developing new treatments for cardiac diseases and neurological disorders.

What are the specific requirements for the storage and maintenance of the Human HCN4 Stable Cell Line-HEK293?

A: The cells need to be stored in low-temperature conditions (typically in a liquid nitrogen tank at -196°C) and regularly resuscitated to maintain their biological activity and genetic stability.

How can the stability and consistency of the Human HCN4 Stable Cell Line-HEK293 be ensured during long-term culture?

A: Avoid overgrowth and cross-contamination of cells, and regularly perform phenotypic and genotypic testing.

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