Cat.No. : CSC-RI0102 Host Cell: HEK293
| Cat. No. | CSC-RI0102 |
| Description | This cell line is engineered to overexpress human HCN4. |
| Background | The human HCN4 gene encodes the pore-forming subunit of a hyperpolarization-activated, cyclic nucleotide-modulated cation channel. HCN4 channels are the predominant HCN isoform in the sinoatrial node and contribute to pacemaker current that controls rhythmic activity in the heart and brain. The HCN4 therapeutic potential includes treatment of cardiac arrhythmia and ischemic heart disease. |
| Gene | HCN4 |
| Gene Species | Homo sapiens (Human) |
| Alias | HCN4, SSS2 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The cell line was established by stably transfecting the human HCN4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) gene into HEK293 cells.
A: It is primarily used to study the role of HCN4 in cardiac rhythm regulation and arrhythmias.
A: It can be used to study the role of HCN4 in neural signal transduction and the regulation of neuronal excitability.
A: This cell line is suitable for screening drugs that affect HCN4 channel activity, important for developing new treatments for cardiac diseases and neurological disorders.
A: The cells need to be stored in low-temperature conditions (typically in a liquid nitrogen tank at -196°C) and regularly resuscitated to maintain their biological activity and genetic stability.
A: Avoid overgrowth and cross-contamination of cells, and regularly perform phenotypic and genotypic testing.
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