Cat.No. : CSC-RO0013 Host Cell: CHO-K1
| Cat. No. | CSC-RO0013 |
| Description | This cell line is engineered to stably overexpress the human HAVCR2 in CHO-K1 cells. |
| Background | The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. |
| Gene | HAVCR2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | HAVCR2 hepatitis A virus cellular receptor 2 [ Homo sapiens ] |
| Gene Symbol | HAVCR2 |
| Synonyms | HAVCR2; hepatitis A virus cellular receptor 2; FLJ14428; Tim 3; TIM3; TIMD3; kidney injury molecule-3; T-cell membrane protein 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; KIM-3; Tim-3; TIMD-3; HAVcr-2; |
| Gene ID | 84868 |
| UniProt ID | Q8TDQ0 |
| mRNA Refseq | BC063431 |
| Chromosome Location | 5q34 |
| MIM | 606652 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human HAVCR2 Stable Cell Line - CHO is likely derived from CHO (Chinese Hamster Ovary) cells and has been genetically modified to stably express the human HAVCR2 gene. The establishment of this stable cell line typically involves transfecting CHO cells with a plasmid or viral vector containing the human HAVCR2 gene and a selectable marker, followed by the selection of cells expressing HAVCR2. This results in the creation of a stable cell line capable of consistently producing the HAVCR2 protein.
A: The Human HAVCR2 Stable Cell Line - CHO can be used in various scientific research applications, particularly in the study of human HAVCR2, also known as Tim-3. Some potential applications include investigating the role of HAVCR2 in immune regulation, immune checkpoint pathways, and its interaction with ligands such as galectin-9. Researchers can use this cell line to study HAVCR2-mediated signaling, immune responses, and the development of therapeutics targeting the HAVCR2 pathway.
A: The Human HAVCR2 Stable Cell Line - CHO may offer specific advantages, such as stable and consistent expression of human HAVCR2. CHO cells are a widely used expression system known for their ability to produce recombinant proteins effectively. This cell line could be advantageous for researchers interested in studying human HAVCR2 in a controlled and reproducible environment. However, the choice of cell line should align with the specific research goals and experimental requirements.
A: To maintain the Human HAVCR2 Stable Cell Line - CHO, researchers should typically culture the cells in a suitable growth medium for CHO cells, supplemented with any necessary selection agents or antibiotics. The specific culture conditions, including temperature, CO2 concentration, and passaging protocols, should be in accordance with established protocols provided by the cell line provider or described in relevant literature.
A: When working with the Human HAVCR2 Stable Cell Line - CHO, researchers should consider the following precautions: a. Regularly monitor and maintain the stability of human HAVCR2 expression to ensure consistent results.
b. Confirm the presence of the selection agent or antibiotic to prevent the loss of HAVCR2-expressing cells.
A: Researchers should be aware of potential limitations when using the Human HAVCR2 Stable Cell Line - CHO. One important consideration is the possibility of differences in post-translational modifications or glycosylation patterns compared to native human cells. These modifications can impact the function and binding affinity of HAVCR2. Validation experiments and comparisons with native human cells may be necessary to ensure that findings from this cell line accurately represent the biology of human HAVCR2.
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