Human ASIC3 Stable Cell Line-HEK293

Name: Human ASIC3 Stable Cell Line-HEK293
SKU: CSC-RI0015

Cat.No. : CSC-RI0015 Host Cell: HEK293

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RI0015
Description	This cell line is engineered to overexpress human ASIC3.
Gene	ASIC3
Gene Species	Homo sapiens (Human)
Alias	ASIC3, ACCN3, DRASIC, SLNAC1, TNaC1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Publications

Q & A

Customer Reviews

Acid-sensing ion channels (ASICs) are structurally related to epithelial sodium channel proteins. They are gated by protons, blocked by amiloride, and their transcripts are localized to sensory ganglia. Their pH sensitivity and distribution makes them interesting candidates to serve as primary transducers of local pH changes during ischemia or tissue injury to pain signals to the CNS. These channels can allow quite large and persistent inward currents in native sensory neurons that can cause sustained firing of action potentials that lead to pain perception. Using the rat ASIC3 sequence de Weille et al. cloned the human gene encoding ASIC3. During severe cardiac ischemia extracellular pH levels can drop as low as 6.7, and a study of eight different functional properties of large acid-gated currents in rat cardiac afferent neurons corresponded well to ASIC3 channels transfected into COS-7 cells.

Customer Q&As

What is the source of Human ASIC3 Stable Cell Line-HEK293, and how was it established?

A: Human ASIC3 Stable Cell Line-HEK293 is established by stably transfecting the human ASIC3 gene into HEK293 cells.

What are the cell culture conditions for Human ASIC3 Stable Cell Line-HEK293, including requirements for culture medium and culture temperature?

A: Human ASIC3 Stable Cell Line-HEK293 is typically maintained in cell culture conditions with appropriate culture medium, usually at a temperature of around 37°C. The specific formulation of the culture medium and culture conditions can be optimized according to research needs.

What are the key parameters to consider when conducting electrophysiological experiments using Human ASIC3 Stable Cell Line-HEK293?

A: Key parameters to consider when conducting electrophysiological experiments include the type of electrode used for membrane potential recordings, the equipment and settings for membrane potential measurements, and the stability of the cells. Ensuring the accuracy of these parameters is crucial for reliable experimental results.

What are some specific application cases of Human ASIC3 Stable Cell Line-HEK293 in neurosignal transmission research?

A: This cell line can be used in neurosignal transmission research to investigate the role of ASIC3 channels in processes such as pain perception and neurotransmitter release. Existing literature references its applications in the field of neurobiology.

How is the activity of ASIC3 channels assessed in drug screening using Human ASIC3 Stable Cell Line-HEK293?

A: The assessment of ASIC3 channel activity in drug screening is typically conducted through techniques such as electrophysiological experiments to record channel activity and calcium imaging. The impact of drugs can be evaluated through these experiments.

When conducting research using Human ASIC3 Stable Cell Line-HEK293, how should potential cell toxicity or adaptation issues be addressed?

A: Addressing potential cell toxicity or adaptation issues may require optimizing culture conditions, selecting suitable culture media, and adding appropriate cell factors to ensure the health and adaptability of the cells.

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