Human ACCN3 Stable Cell Line-CHO

Name: Human ACCN3 Stable Cell Line-CHO
SKU: CSC-RI0065
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0065

Host Cell : CHO Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0065
Description	This cell line is engineered to overexpress human ASIC3.
Background	The human ACCN3 gene encodes the pore-forming subunit of ASIC3, a proton-activated, Na+-permeable channel. ASIC3 channels expressed primarily in dorsal root ganglion neurons are therapeutic targets in pain and inflammation.
Target Gene	ASIC3
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuACCN3
Alias	ASIC3, ACCN3, DRASIC, SLNAC1, TNaC1
Host Cell	CHO
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ASIC3 acid-sensing (proton-gated) ion channel 3 [ Homo sapiens ]
Gene Symbol	ASIC3
Synonyms	ACCN3; TNaC1; DRASIC; SLNAC1
GeneID	171209
Uni ProtID	Q9UHC3
mRNA Refseq	NM_004769.3
Protein Refseq	NP_004760.1
Chromosome Location	7q35
Function	PDZ domain binding; cation channel activity; enterobactin transporter activity; ligand-gated sodium channel activity; sodium channel activity;
MIM	611741

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