Stranded mRNA-seq Sample Prep Kit

Name: Stranded mRNA-seq Sample Prep Kit
SKU: DSPK-10
Rating: 4.0 (4 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

DSPK-10

Description

Stranded mRNA-seq Sample Prep Kit is designed for the preparation of stranded transcriptome libraries for next generation sequencing (NGS) platforms of Illumina. Compared with the universal transcriptome library prep kits, this kit enables the insertion of dUTP during the cDNA 2nd strand synthesis reaction. The double-strand cDNA is digested with uracil-DNA glycosylase (UDG) to remove the second strand containing dUTP before PCR enrichment so that only the information of the first strand is retained. With this kit, you can obtain both standard transcriptome information and strand-specific information from NGS data (e.g. from sense or antisense DNA).

Features

• Suitable for strand-specific mRNA-seq
• Suitable for RNA samples of eukaryotes such as animals, plants, fungi and so on
• Using truncated RNA adapters which exhibit better ligation efficiency and fewer adapter dimmers
• High quality and reliable performance

Sample Type

RNA samples from eukaryotes

Storage

• Box-1: 2-8 °C; Do NOT freeze the Oligo (dT)25 Capture Beads.
• Box-2 & Box-3: -20 °C.

Publications

Q & A

Customer Reviews

Customer Q&As

What is stranded RNA-seq?

A: Stranded RNA-seq, (also known as directional RNA-seq), is a technique used in genomics that allows the measurement of gene expression while simultaneously preserving the information about the direction of transcription. Essentially, it can be used to show which way the RNA is transcribed on the DNA strand, resulting in a much more detailed and a more accurate description of the transcriptome.

What is the difference between stranded and unstranded RNA-Seq?

A: Read ambiguity in stranded RNA-seq arises only from overlapping genes transcribed from the same strand. In contrast, for non-strand RNA-seq, ambiguity arises from both overlapping genes on the same strand and overlapping genes on the opposite strand.

What are the advantages of stranded RNA-Seq?

A: RNA sequencing strands allow researchers to determine which DNA strand (sense or antisense) a transcript came from. Compared with regular RNA sequencing methods, strand RNA sequencing can discover novel transcripts, distinguish transcripts from overlapping genes, find antisense sequences, and annotate genes.

What is the difference between total RNA and mRNA sequencing in sequencing reads?

A: If only mRNA information is required, then mRNA-Seq offers greater read depth at lower cost than total RNA-Seq. This is because sequencing reads (typically 25–50 million reads per sample) are focused on poly(A)-enriched RNA molecules.

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Customer Reviews

Identification of more genes

Stranded mRNA-seq Sample Prep Kits are optimized for the improved coverage of GC-rich and low-abundance transcripts, resulting in the identification of more genes.

United States

08/23/2020

Worked very well

High uniformity across varying amounts of sample input. Good experimental results were obtained.

United States

05/12/2021

Great!

With similar numbers of filter-passed reads, Creative Biogene's Stranded mRNA-Seq libraries generate a greater percentage of mapped reads and lower duplication rates than analogous libraries prepared with the other Stranded mRNA-Seq Sample Prep Kit while maintaining 99% strand-specificity.

France

08/15/2021

Invaluable tool

GLTPD1, a lesser-expressed transcript, is covered more comprehensively with the Creative Biogene's Stranded mRNA-seq Sample Prep Kit at 500 ng input total RNA.

United States

10/09/2021

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Related Products

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Stranded mRNA-seq Sample Prep Kit

Publications

Q & A

Customer Reviews

What is stranded RNA-seq?

What is the difference between stranded and unstranded RNA-Seq?

What are the advantages of stranded RNA-Seq?

What is the difference between total RNA and mRNA sequencing in sequencing reads?

Identification of more genes

Worked very well

Great!

Invaluable tool

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