Universal mRNA-seq Sample Prep Kit

Name: Universal mRNA-seq Sample Prep Kit
SKU: DSPK-09
Rating: 4.0 (2 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

DSPK-09

Description

Universal mRNA-seq Sample Prep Kit is designed for the preparation of transcriptome libraries for next generation sequencing (NGS) platforms of Illumina. The kit includes all of the enzymes and buffers for poly (A) mRNA enrichment and mRNA-seq library construction. Universal mRNA-seq Sample Prep Kit can be used to construct RNA sequencing libraries of eukaryotic samples such as animals, plants, fungi, etc.

Features

• Suitable for RNA samples of eukaryotes such as animals, plants, fungi and so on
• Using truncated RNA adapters which exhibit better ligation efficiency and fewer adapter dimmers
• High quality and reliable performance
• High library yield, which can meet variety of experimental needs

Sample Type

RNA samples from eukaryotes

Storage

• Box-1: 2-8 °C; Do NOT freeze the Oligo (dT)25 Capture Beads.
• Box-2 & Box-3: -20 °C.

Publications

Q & A

Customer Reviews

Customer Q&As

How can I improve my RNA purity?

A: High-quality total RNA can be recovered from any sample type by keeping three simple tips in mind: ensuring the sample is stable after collection, lysing the sample thoroughly and thoroughly, and eliminating any potential DNA contamination.

How much RNA do you need for library prep?

A: For standard RNA-seq library preparation, you should aim to start with between 100 ng to 1 μg of purified RNA.

What is the difference between total RNA and mRNA sequencing?

A: Total RNA samples can contain up to 90% ribosomal RNA (rRNA) sequences, which are not informative for transcriptome or gene expression studies, while mRNA typically only represents 1% to 2% of total RNA. Therefore, it is highly desirable to enrich the mRNA in the sample.

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Customer Reviews

High efficiency

This kit can efficiently isolates mRNA and detects most coding genes.

United States

04/11/2023

Save samples

Great! Highly sensitive mRNA detection with input as low as 5 ng of total RNA.

Germany

08/09/2023

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Related Products

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Universal mRNA-seq Sample Prep Kit

Publications

Q & A

Customer Reviews

How can I improve my RNA purity?

How much RNA do you need for library prep?

What is the difference between total RNA and mRNA sequencing?

High efficiency

Save samples

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