pcDNA3.1(+)

Name: pcDNA3.1(+)
SKU: VET1450
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : VET1450

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Product Information

Cat. No.	VET1450
Background	pCDNA3.1(+) is a 5.4 kb vector derived from pCDNA3 designed for high-level stable and transient expression in mammalian hosts. Most mammalian cells are capable of high levels of stable and non-replicative transient expression. Human cytomegalovirus immediate early (CMV) promoter for high-level expression in a wide range of mammalian cells. Multiple cloning sites in forward (+) and reverse (-) directions to facilitate cloning of neomycin resistance genes for selection of stable cell lines. Aberrant replication in cell lines latently infected with SV40 or expressing the SV40 large T antigen (e.g., COS-1, COS-7).
Host Cell	Mammalian cells, Escherichia coli
Promoter	CMV
Resistance	Ampicillin
Selection	Neomycin
Vector Type	Expression Vector
Vector Size	5428 bp

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Applications

Q & A

Customer Reviews

The pcDNA3.1(+) plasmid is a widely used expression vector in molecular biology and genetic engineering research. Its numerous applications include:

1. Cloning and expression of target genes: pcDNA3.1(+) enables the cloning of target genes of interest into the multiple cloning site (MCS) within the plasmid. The inserted gene can be expressed under the control of a strong constitutive promoter, such as the cytomegalovirus (CMV) promoter, allowing researchers to produce large amounts of the protein encoded by the gene.

2. Gene overexpression studies: By expressing a gene of interest using pcDNA3.1(+), researchers can study the effects of gene overexpression on cellular processes, pathways, or phenotypes.

3. Construction of reporter gene vectors: pcDNA3.1(+) can be used to construct reporter gene vectors for monitoring gene expression or protein function.

4. Vaccine and therapeutic protein production: The pcDNA3.1(+) vector can be used for the production of recombinant proteins, including vaccines or therapeutic proteins. It offers a system for the large-scale production of proteins, allowing for potential use in biopharmaceutical applications.

Customer Q&As

Which enzyme is recommended for linearizing pcDNA3.1 vectors to produce stable transfectants?

A: Although not required, if you want to linearize the pcDNA3.1 vector before stable transfection, Pvu I or Sca I are single-cutting enzymes that cut within the ampicillin resistance gene and should work well.

What are pcDNA3.1(+) vector derived from?

A: They are derived from pcDNA3 vector.

In which type of cells can pcDNA3.1(+) vector carry out high-level stable and non-replicative transient expression?

A: It can carry out high-level stable and non-replicative transient expression in most mammalian cells.

What is the difference between pcDNA3.1 vector and pcDNA3.3-TOPO vector?

A: The pcDNA3.1 vector contains the core CMV promoter that is truncated before the initiation of transcription, while the pcDNA 3.3-TOPO vector contains 672 bp of the native CMV promoter.

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Customer Reviews

High-level expression

pcDNA3.1(+) enables high-level expression in a wide range of mammalian cells. This has greatly enhanced the overall value of our research.

Germany

2022-01-14

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pcDNA3.1(+)

Product Information

Applications

Q & A

Customer Reviews

Which enzyme is recommended for linearizing pcDNA3.1 vectors to produce stable transfectants?

What are pcDNA3.1(+) vector derived from?

In which type of cells can pcDNA3.1(+) vector carry out high-level stable and non-replicative transient expression?

What is the difference between pcDNA3.1 vector and pcDNA3.3-TOPO vector?

High-level expression

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