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pcDNA3.2/V5-DEST

For research use only. Not intended for any clinical use.
Cat.No.
VET1312
Host Cell
mammalian cells, Escherichia coli
Promoter
CMV
Resistance
Ampicillin
Selection
Neomycin
Vector Size
7711 bp
Vector Type
Expression Vector

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Q & A

Customer Reviews

Customer Q&As
What is the system of this vector, and whether it can be used alone?

A: pcDNA3.2/V5-DEST Is the Gateway system carrier, which cannot be used alone.

How will the vector be used?

A: It needs to be used together with the donor carrier and the entry carrier.

Can this empty vector be used in any E. coli species?

A: On the empty vector, there is a ccdB expression box after the CMV promoter, so this empty vector can only be replicated and amplified in a dedicated E. coli such as DB3.1. When using this vector, please pay attention to the corresponding DB3.1 receptor.

Is there a universal primer for detecting the recombinant gene on this vector?

A: There are universal primers to detect positive clones that can be used to:T7 Fwd: 5'[TAATACGACTCACTATAGGG]3'

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Customer Reviews
A Quality Product

I used this vector to get a high proportion of positive clones and a very pure background.

United States

11/04/2022

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