pCAMBIA1201

Abiotic stresses such as drought and high salinity generate excessive reactive oxygen species (ROS) in plants, leading to oxidative damage and programmed cell death. The researchers investigated the functional role of rice peroxygenase OsPXG9 in modulating oxylipin metabolism and ROS scavenging. Using pCAMBIA1201/OsPXG9 overexpression vectors constructed with Creative Biogene's pCAMBIA1201 backbone, OsPXG9 was ectopically expressed in rice seedlings. Subsequent phenotypic and biochemical analyses demonstrated that OsPXG9 overexpression reduced ROS accumulation, mitigated drought- and salt-induced cell death, and selectively enhanced stress-induced antioxidant pathways, highlighting its mechanistic role in plant resilience against abioticstress.

Figure 1. DAB, NBT, and Evan's blue staining revealed lower ROS accumulation and reduced cell death in OsPXG9-overexpressing rice seedlings compared with wild-type under drought and salt stress. (Tran AD, et al., 2025)