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pTT5

For research use only. Not intended for any clinical use.
Cat.No.
VET1182
Promoter
CMV
Selection
Ampicillin
Source
Escherichia coli
Species
Bacterial

Background

Case Study

Applications

Publications

Q & A

Customer Reviews

The pTT5 vector is a suitable vector for both bacterial cloning as well as protein expression in mammalian hosts. The prominent properties of this plasmid are the CMV promoter, the PMB1 ori replicon, and the beta globin poly (A) signal terminator, which enhance its ability to stimulate protein over-expression in the host system. The CMV promoter is a constitutive promoter that guarantees robust gene expression. This makes it applicable for the high-level expression of recombinant proteins. On the other hand, the PMB1 ori replicon assists DNA replication thereby ensuring the stability of the plasmid in the host cell. The pTT5 vector also incorporates the beta globin poly (A) terminator. This signal is responsible for ending the transcription process correctly, ensuring that the mRNA transcript carries the complete information of the gene in focus. In terms of resistance, the plasmid carries an ampicillin resistance gene, which implies that only bacteria incorporating the vector will be able to survive in ampicillin enriched medium.

Rapid generation of high-quality target antibodies is critical for studies employing immunoassays. The use of recombinant antibody technology that relies on genetic engineering is one of the means to produce high-quality antibodies. In this study, the researchers obtained the protein sequence of a variable region of a 17 β-estradiol (E2) antibody from the Protein Data Bank (PDB) and subsequently constructed heavy (H) and light (L) chain expression vectors through codon optimization. Immunoglobulin G (IgG), antigen-binding fragment (Fab) and single-chain variable fragment (scFv) antibodies were transiently expressed, purified and characterized respectively. Further compare the effects of different expression vectors on IgG antibody expression. Among them, the expression yield based on pTT5 vector was the highest, reaching 27 mg/L.

SDS-PAGE of the purified recombinant antibody [IgG (A), Fab (B), and scFv (C)] of 17 Beta-estradiol based on the pTT5 vector.Figure 1. SDS-PAGE of the purified recombinant antibody [IgG (A), Fab (B), and scFv (C)] of 17 β-estradiol based on the pTT5 vector. (Lu, Xin, et al., 2023)

The pTT5 vector is a versatile tool in molecular biology and biotechnology with several key applications: Protein Expression: pTT5 is often used to facilitate high-level expression of recombinic proteins in various host systems. It allows the target protein to get expressed and greatly simplifies the purification process. Vaccine Development: In recent studies, pTT5 vector has been employed in the construction of subunit vaccines, where it aids in producing protein antigens in large quantities. This approach can increase the efficacy of the vaccine and reduce potential side effects. Therapeutic Protein Production: The vector is utilized for the manufacturing of therapeutic proteins. It can be engineered to produce proteins of interest, which can then be purified and used in different therapeutic applications. Gene Therapy: Gene therapy techniques often use vectors like pTT5 to deliver specific genes into patient cells. This can help to treat genetic disorders by replacing a disease-causing gene with a healthy copy or introducing a new gene to help fight a disease.
Customer Q&As
What studies are the pTT 5 carriers commonly used for?

A: The pTT5 vectors are often used for the expression of antibody light or heavy chains in mammalian cells.

What should we pay attention to when expressing the genes constructed from this plasmid?

A: Gene expression using this plasmid construct was a nonfusion expression, requiring the addition of a Kozak sequence to the front of the gene during plasmid construction to increase protein expression efficiency.

What is the purpose of pTT5 vector?

A: The pTT5 vector is a suitable vector for bacterial cloning and protein expression in mammalian hosts.

What promoter does the pTT5 vector use for robust expression?

A: The pTT5 vector utilizes a CMV promoter to drive robust expression.

How does the pTT5 vector facilitate replication in human embryonic kidney (HEK) 293-6E cells?

A: The pTT5 vector contains an oriP cis-element, which assists in replication in HEK 293-6E cells.

Does the pTT5 vector have specific advantages in protein expression in mammalian hosts?

A: Yes, the pTT5 vector is designed to facilitate high levels of protein expression in mammalian hosts.

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Customer Reviews
Reliable

The pTT5 vectors are good transient expression carriers for mammalian cell proteins.

United States

04/06/2023

Obtain high-quality products

The pTT5 vector has greatly improved the efficiency of protein processing and secretion compared to the pBSSK vector. This has allowed us to obtain high-quality products.

United Kingdom

08/15/2021

Meaningful results

By combining gene dosing with mild hypothermic expression conditions and using pTT5 as a carrier DNA, we have achieved a significant improvement in the quality of our expressed proteins. This has greatly enhanced the overall value of our research.

United States

10/09/2021

Reliable

My experience with the transfection efficiency of pTT5 has been exceptional, consistent, and reliable. It's been a great tool in the easy detection of proteins, both in mammalian cells and other biological systems.

United Kingdom

01/10/2024

Perfect for my research requirements

The pTT5 vector's multiple cloning sites allow a wide range of foreign genes to be cloned and expressed, making it incredibly adaptable to my research requirements.

French

05/17/2020

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