Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : OVT2710
| Cat. No. | OVT2710 |
| Product Type | Vector |
| Host Cell | Mammalian cells, Escherichia coli |
| Promoter | U6 |
| Resistance | Ampicillin |
| Vector Size | 5721 bp |
Chronic alcohol consumption induces hepatic lipid accumulation, oxidative stress, and inflammation, contributing to alcohol-associated liver disease (ALD). The researchers investigated the role of the ubiquitin ligase adaptor FAF2 in ALD pathogenesis. Using liver-specific knockdown via adeno-associated virus serotype 8 (AAV8) carrying Creative Biogene's pAAV-ZsGreen-FAF2-shRNA, they demonstrated that suppression of FAF2 mitigates ethanol-induced liver steatosis in mice. Transcriptomic analyses revealed that FAF2 knockdown altered expression of lipid metabolism genes regulated by SREBP1 and the FOXO3-SIRT6-Pcsk9 axis, while enhancing adipose triglyceride lipase activity via upregulation of Cgi-58 and downregulation of Elmod2. These molecular changes collectively reduce hepatic lipid accumulation, lower plasma LDL cholesterol, and alleviate alcohol-induced hepatic injury.
Figure 1. AAV8-mediated FAF2 knockdown reduced hepatic steatosis in ethanol-fed mice, as shown by H&E and Oil Red O staining, and decreased plasma AST, ALT, triglyceride, and cholesterol levels. (Huda N, et al., 2025)
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