Cat.No. : CSC-RO0911 Host Cell: CHO-K1
| Cat. No. | CSC-RO0911 |
| Description | This cell line is engineered to stably overexpress human TREM1-TYROBP (also named as TREM1-DAP12). |
| Gene | TREM1-TYROBP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
HIV and hepatitis C virus (HCV) chronically infect approximately 35 million and 75 million people globally, respectively. Researchers investigated how HIV and HCV infections trigger inflammation in liver cells, potentially leading to diseases like Hepatocellular Carcinoma (HCC). They found that HIV and HCV stimulate innate immune responses in liver-resident macrophages, including increased expression of Triggering Receptor Expressed on Myeloid cells 1 (TREM1). TREM1 amplifies immune signaling, exacerbating inflammation. Targeting TREM1 signaling could offer therapeutic opportunities to reduce HIV-induced liver inflammation.
Figure 1. A stable cell line overexpressing TREM1 was generated by researchers through lentiviral transduction of THP1 cells to assess the function of TREM1 signaling during viral exposure. TREM1 mRNA expression was confirmed by qPCR analysis, and cell surface expression was validated using flow cytometry. (Hyun J, et al., 2019)
A: HEK293T cells were likely selected for their high transfection efficiency and suitability for stable transgene expression, facilitating the study of NECTIN4 biology and its role in cell adhesion, signaling, and cancer progression.
A: Stability was likely assessed through methods such as flow cytometry, immunoblotting, or functional assays measuring NECTIN4-mediated signaling, with continuous selection pressure applied.
A: Characterization may involve analysis of NECTIN4 membrane localization, interaction with binding partners such as nectins or afadin, downstream signaling pathways, and functional implications in cell adhesion, migration, or cancer metastasis.
A: Quality control likely included confirmation of NECTIN4 expression levels, validation of its functional activity, assessment of off-target effects, and validation of phenotypic changes associated with NECTIN4 modulation.
A: Comparative analysis with primary epithelial cells or in vivo models helps validate the relevance of NECTIN4 expression in cancer initiation, progression, and metastasis, guiding the development of NECTIN4-targeted therapeutics for cancer treatment.
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Unmatched consistency! The Human TREM1-TYROBP Stable Cell Line in CHO-K1 cells delivers reliable TREM1-TYROBP expression, ensuring robust results in my immunology research.
Empowering advanced studies! With stable TREM1-TYROBP expression, I can explore innate immune responses and inflammation mechanisms with confidence, driving scientific breakthroughs.
Exceptional performance! This cell line exceeds expectations, providing a solid foundation for investigating TREM1-TYROBP-targeted therapies and immune modulation strategies.
Streamlining research processes! Its stable expression streamlines experimental workflows, enhancing efficiency and enabling in-depth data analysis with ease.
An indispensable asset! The Human TREM1-TYROBP Stable Cell Line has revolutionized my research, offering valuable insights into TREM1-TYROBP-mediated immune signaling and potential therapeutic interventions.
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