Human TREM1-TYROBP Stable Cell Line - HEK293T

Name: Human TREM1-TYROBP Stable Cell Line - HEK293T
SKU: CSC-RO0893
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0893

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0893
Description	This cell line is engineered to stably overexpress human TREM1-TYROBP (also named as TREM1-DAP12).
Target Gene	TREM1-TYROBP
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What role does the Human TREM1-TYROBP Stable Cell Line - HEK293T play in inflammatory responses?

A: The Human TREM1-TYROBP Stable Cell Line - HEK293T is primarily used for studying inflammatory responses. The stable co-expression of TREM1 (Triggering Receptor Expressed on Myeloid cells 1) and TYROBP (TYRO protein tyrosine kinase-binding protein) in HEK293T cells can simulate the activation of immune cells and the conduction of inflammatory signals. This helps in understanding the mechanisms of immune responses and inflammation-related diseases.

How does the interaction between TREM1 and TYROBP in the Human TREM1-TYROBP Stable Cell Line - HEK293T affect cell signaling?

A: In the Human TREM1-TYROBP Stable Cell Line - HEK293T, the interaction between TREM1 and TYROBP leads to the activation of immune receptors, which in turn triggers downstream signaling pathways like NF-κB activation. This interaction is crucial for the regulation of inflammation and immune responses, especially in the study of autoimmune and inflammatory diseases.

What disease models can the Human TREM1-TYROBP Stable Cell Line - HEK293T be used for research?

A: This cell line can be used in research for various disease models, especially those involving inflammation and immune responses, such as autoimmune diseases, infectious diseases, certain types of cancer, and neurodegenerative diseases.

What key factors should be considered when using the Human TREM1-TYROBP Stable Cell Line - HEK293T for drug screening?

A: When using this cell line for drug screening, it is important to consider the drug's impact on the TREM1-TYROBP signaling pathway, cytotoxicity, drug specificity and selectivity, as well as the drug's effect on cell functions like cytokine release and gene expression.

How does the Human TREM1-TYROBP Stable Cell Line - HEK293T perform in terms of cytokine release?

A: Due to the key role of TREM1 and TYROBP in immune responses, the Human TREM1-TYROBP Stable Cell Line - HEK293T shows significant release of cytokines like IL-1β, TNF-α under certain stimuli, which is very important for studying inflammation responses and the effects of related drugs.

How can gene editing techniques be used to further modify the Human TREM1-TYROBP Stable Cell Line - HEK293T for studying specific pathological mechanisms?

A: Through gene editing technologies like CRISPR/Cas9, the Human TREM1-TYROBP Stable Cell Line - HEK293T can be further modified, such as by knocking out or inserting specific genes, to study the roles of TREM1 and TYROBP in specific pathological mechanisms.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *