Cat.No. : CSC-RO0893 Host Cell: HEK293T
| Cat. No. | CSC-RO0893 |
| Description | This cell line is engineered to stably overexpress human TREM1-TYROBP (also named as TREM1-DAP12). |
| Gene | TREM1-TYROBP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human TREM1-TYROBP Stable Cell Line - HEK293T is primarily used for studying inflammatory responses. The stable co-expression of TREM1 (Triggering Receptor Expressed on Myeloid cells 1) and TYROBP (TYRO protein tyrosine kinase-binding protein) in HEK293T cells can simulate the activation of immune cells and the conduction of inflammatory signals. This helps in understanding the mechanisms of immune responses and inflammation-related diseases.
A: In the Human TREM1-TYROBP Stable Cell Line - HEK293T, the interaction between TREM1 and TYROBP leads to the activation of immune receptors, which in turn triggers downstream signaling pathways like NF-κB activation. This interaction is crucial for the regulation of inflammation and immune responses, especially in the study of autoimmune and inflammatory diseases.
A: This cell line can be used in research for various disease models, especially those involving inflammation and immune responses, such as autoimmune diseases, infectious diseases, certain types of cancer, and neurodegenerative diseases.
A: When using this cell line for drug screening, it is important to consider the drug's impact on the TREM1-TYROBP signaling pathway, cytotoxicity, drug specificity and selectivity, as well as the drug's effect on cell functions like cytokine release and gene expression.
A: Due to the key role of TREM1 and TYROBP in immune responses, the Human TREM1-TYROBP Stable Cell Line - HEK293T shows significant release of cytokines like IL-1β, TNF-α under certain stimuli, which is very important for studying inflammation responses and the effects of related drugs.
A: Through gene editing technologies like CRISPR/Cas9, the Human TREM1-TYROBP Stable Cell Line - HEK293T can be further modified, such as by knocking out or inserting specific genes, to study the roles of TREM1 and TYROBP in specific pathological mechanisms.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
