Human CDK4 Knockout Cell Line-HCT116

Cancer Research: This cell line is useful for studying the role of CDK4 in cancer cell proliferation. By comparing the proliferation rates of CDK4 knockout cells to wild-type HCT116 cells, researchers can better understand how CDK4 promotes tumor growth, potentially identifying new targets for cancer treatment. Drug Development: The CDK4 knockout HCT116 cell line can be used as a model for screening and evaluating CDK4 inhibitors and other therapeutic agents. It provides insight into the efficacy and specificity of potential drugs, allowing researchers to identify compounds that specifically target the CDK4 pathway without affecting other cellular mechanisms. Cell Cycle Regulation Studies: CDK4 plays a critical role in the cell cycle, specifically the transition from G1 to S phase. This knockout cell line is useful for elucidating the molecular mechanisms involved in cell cycle regulation and determining how CDK4 loss affects cell cycle progression. Genomic Studies: This cell line can be used for genetic studies, including studying gene regulation, expression, and the effects of CDK4 loss on the broader genomic landscape. It can be used to study compensatory mechanisms that cells may employ in the absence of CDK4. Signal Transduction Studies: CDK4 is part of several signal transduction pathways. By using CDK4 knockout cell lines, researchers can dissect these pathways and understand how the loss of CDK4 affects various signaling cascades.