EF1a-hCDK4(RFP, Bla) Lentiviral Particles

EF1a-hCDK4(RFP, Bla) lentiviral particles provide a powerful and versatile tool for gene manipulation in mammalian cells. Modified with the EF1a promoter, this lentivirus ensures stable and sustained expression of the human CDK4 gene in a variety of cell types, including primary cells and those difficult to transfect. Its included red fluorescent protein (RFP) reporter gene allows for easy visualization and tracking of transduced cells via fluorescence microscopy or flow cytometry, enabling real-time monitoring without additional staining or fixation. Furthermore, the blast fungicide resistance gene (Bla) provides a highly efficient screening mechanism, allowing researchers to remove untransduced cells under blast fungicide treatment, thereby establishing stable cell lines. This dual-function system, combining a strong promoter, a visual reporter gene, and a selection marker, ensures high transduction efficiency, reliable gene expression, and a simplified workflow.

The primary application of EF1a-hCDK4(RFP, Bla) lentiviral particles is cell immortalization, particularly suitable for primary human cells that typically age after limited passages. By overexpressing CDK4 (a key regulator of the G1/S phase transition in the cell cycle), this lentivirus enables cells to bypass replicative senescence, thereby extending their lifespan and facilitating long-term research. Furthermore, the lentiviral particles play a crucial role in investigating the function of CDK4 in tumorigenesis, drug resistance, and cell cycle dysregulation. The RFP reporter gene allows for precise isolation of transduced cells via fluorescence-activated cell sorting (FACS), resulting in a homogeneous cell population for subsequent proteomics, transcriptomics, or high-throughput screening analyses. Beyond cell immortalization, this tool also supports co-transduction experiments with other lentiviral vectors (e.g., expressing cyclin D or other cell cycle regulators) to mimic complex signaling pathways.