EF1a-hMYC(RFP, Bla) Lentiviral Particles

EF1a-hMYC(RFP, Bla) lentiviral particles are a cutting-edge tool for gene manipulation in mammalian cells. Genetically engineered, they enable efficient and flexible delivery of the human MYC oncogene. The EF1a promoter drives stable MYC gene expression, ensuring consistent protein levels across transduced cell populations. This lentiviral vector integrates two key selection markers: a red fluorescent protein (RFP) reporter gene for real-time monitoring of transduction efficiency via fluorescence microscopy or flow cytometry; and a blastcinidyl resistance gene (Bla) for stable selection of transduced cells in culture. The lentiviral platform itself offers significant advantages, including the ability to infect both dividing and non-dividing cells, long-term sustained transgene expression through genome integration, and broad tropism across multiple cell types.

The primary application of EF1a-hMYC(RFP, Bla) lentiviral particles is in cell immortalization, where overexpression of MYC can suppress senescence pathways, thereby extending the lifespan of primary cells or difficult-to-culture cell lines. This is of significant value for establishing stable cell models from patient-derived samples, primary epithelial cells, or stem cells (which have limited in vitro proliferative capacity). Researchers can rapidly generate immortalized cell lines by transducing target cells and subsequently screening for blastomycin; RFP markers immediately confirm successful transduction. Beyond immortalization, these particles facilitate MYC-focused tumorigenesis research, enabling the investigation of MYC-driven signaling pathways, metabolic reprogramming, and tumorigenesis in cancer models. The dual-selection markers also allow for synergistic experiments with other genetic elements or drug therapies. Furthermore, the resulting cell lines can serve as a stable platform for high-throughput drug screening, functional genomics, and disease modeling—particularly in areas where MYC dysregulation plays a pathophysiological role, such as oncology, regenerative medicine, and aging research.