EF1a-hMYC(GFP, Puro) Lentiviral Particles

EF1a-hMYC(GFP, Puro) lentiviral particles represent a cutting-edge tool for gene manipulation in cell biology research. This genetically engineered lentiviral vector system expresses the human MYC oncogene under the control of a highly efficient EF1a promoter, ensuring stable and sustained expression in transduced cells. The vector integrates two key selection markers: a GFP reporter gene for real-time visualization of transduction efficiency and cell localization; and a puromycin resistance gene for rapid and stable screening of successfully transduced cells. Utilizing lentiviral delivery technology, the system achieves high transduction efficiency in both dividing and non-dividing cell types, and stable genome integration enables long-term transgene expression. Its self-inactivation (SIN) design minimizes the risk of insertional mutations while maintaining robust promoter activity.

This lentiviral particle system is particularly important in cell immortalization research because sustained MYC expression can bypass the senescence mechanisms of primary cells. By extending the replication life of previously short-lived cell cultures, researchers can establish stable cell lines for long-term experiments in cancer biology, regenerative medicine, and drug discovery platforms. The GFP reporter gene allows for non-invasive monitoring of transduction success rate and transgene expression dynamics via fluorescence microscopy or flow cytometry, while puromycin screening ensures cell population homogeneity by removing untransduced cells. The system's applications extend to functional genomics research, exploring the role of MYC in cell cycle regulation, apoptosis resistance, metabolic reprogramming, and stem cell maintenance. Furthermore, the system facilitates the development of disease models for tumorigenesis research and provides a platform for screening therapeutics targeting the MYC-driven pathway.