Total RNA Ultrapure Kit

The researchers investigated the latent antibiotic-producing potential of Escherichia coli C91 by activating cryptic biosynthetic gene clusters through ribosome-engineering strategies. Rifampicin-resistant mutants were generated and systematically characterized using antimicrobial activity assays, LC-MS/MS metabolite profiling, genome mining, and transcriptional analysis. In particular, the Total RNA Ultrapure Kit was used to isolate high-quality RNA for qRT-PCR–based transcriptional analysis of siderophore- and antibiotic-associated genes, enabling accurate assessment of gene expression changes in the Δycao mutant relative to the wild-type strain.

Figure 1. qRT-PCR analysis shows the relative expression levels of siderophore- and antibiotic-related genes in the Δycao mutant compared with wild-type E. coli C91, normalized to the housekeeping gene rpoD.(Dashti KM, et al., 2026)

Total RNA Ultrapure Kit provides reliable, high-purity RNA suitable for downstream quantitative gene expression analyses in microbial research. Its performance supports precise transcriptional profiling in studies exploring secondary metabolism, antibiotic biosynthesis, and functional genomics.