Total RNA Ultrapure Kit

Name: Total RNA Ultrapure Kit
SKU: NKR-T019
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : NKR-T019

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Product Information

Cat. No.	NKR-T019
Description	Based on the same principle of Total RNA Kit, the Total RNA Ultrapure Kit is designed for the isolation of total RNA eukaryotic cells orall type of animal and plant tissues. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue samples including difficult-to-lyse fibrous and fatty tissues such as skeletal muscle, heart, brain, adipose tissues and plant, fungal samples. Compared to other standard silica-column procedure, Total RNA Ultrapure Kit provide higher yield and better quality of RNA from all types of tissues.
Sample Type	Universal
Report Gene	Universal
Features	Universal - Suited for all types of biological samples
Applications	Ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Manipulation	High-quality RNA in 60 minutes
Nucleic Acid Type	RNA
Promoter	Phenol/guanidine-base lysis,Silica-based membrane
Selection Marker	RNA
Yield	High Yield
Quality	High purity, purified RNA can be directly used in any downstream applications without the need for further purification.
Regulatory Statement	For Research only.
Storage	All the kit components are guaranteed for at least 12 months from the date of purchase.

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The researchers investigated the latent antibiotic-producing potential of Escherichia coli C91 by activating cryptic biosynthetic gene clusters through ribosome-engineering strategies. Rifampicin-resistant mutants were generated and systematically characterized using antimicrobial activity assays, LC-MS/MS metabolite profiling, genome mining, and transcriptional analysis. In particular, the Total RNA Ultrapure Kit was used to isolate high-quality RNA for qRT-PCR–based transcriptional analysis of siderophore- and antibiotic-associated genes, enabling accurate assessment of gene expression changes in the Δycao mutant relative to the wild-type strain.

Figure 1. qRT-PCR analysis shows the relative expression levels of siderophore- and antibiotic-related genes in the Δycao mutant compared with wild-type E. coli C91, normalized to the housekeeping gene rpoD.(Dashti KM, et al., 2026)

Total RNA Ultrapure Kit provides reliable, high-purity RNA suitable for downstream quantitative gene expression analyses in microbial research. Its performance supports precise transcriptional profiling in studies exploring secondary metabolism, antibiotic biosynthesis, and functional genomics.

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